摘要
巧玲花是我国北方一种极具观赏价值的落叶花灌木,北京百花山具有丰富的巧玲花资源。调查分析表明,其分布在海拔1 200~1 800m范围内。利用ISSR分子标记技术对百花山6个不同海拔梯度(1 200~1 300、1 301~1 400、1 401~1 500、1 501~1 600、1 601~1 700、1 701~1 800m)的巧玲花群体进行遗传多样性分析。结果表明:百花山地区不同海拔巧玲花群体的遗传多样性差异较大,在1 500~1 600m的中海拔地区的群体表现出较高的遗传多样性水平(75.43%),而高海拔和低海拔地区的群体遗传多样性较低(62.82%、73.08%);UPGMA聚类结果表明,遗传距离与海拔呈一定的相关性。相邻海拔的巧玲花群体间遗传距离相对较近,而高山草甸与其他海拔的群体间表现出较远遗传距离,根据群体间的遗传分化系数(Gst)的结果分析,变异主要存在于群体内(89.06%)。综合以上结果认为,海拔的不同引起了生境条件的变化,进而影响巧玲花遗传多样性,遗传多样性水平随海拔的升高呈现低-高-低的分布规律。
Syringapubescens is a deciduous shrub species native to northern China with high potential for ornamental value.There are rich resources of S.pubescens in Baihua Mountain in Beijing,with altitudinal distribution ranging from 1 200 to 1 800 m.Genetic diversity and genetic structure of six different altitude gradient S.pubescens populations(1 200-1 300,1 301-1 400,1 401-1 500,1 501-1 600,1 601-1 700,1 701-1 800m)in Baihua Mountain were studied by ISSR technology.The results showed that the genetic diversity of S.pubescens was higher(75.43 %)in 1 501-1 600 maltitudinal population,while lower genetic diversity was observed in populations of higher and lower altitude(62.82% and 73.08% respectively).The UPGMA cluster analysis indicated that the genetic distances among populations were certainly correlated with their altitudinal distribution.The genetic distance was relative lower between the adjacent altitude of population,however,the genetic distance was relative farther between the alpine meadow and other altitudinal populations.According to the group of the coefficient of genetic differentiation(Gst)analysis,the genetic variation existed in the populations(89.06%).Comprehensively,the genetic diversity of the S.pubescens was affected by the change of the habitat conditions caused by the altitudinal difference,and the distribution of genetic diversity level was low-high-low with the rising of altitude in Baihua Mountain.
出处
《西北林学院学报》
CSCD
北大核心
2016年第4期113-117,共5页
Journal of Northwest Forestry University
基金
国家林木(含竹藤花卉)种质资源平台建设与运行服务(2005DKA21003)
北京市属高等学校高层次人才引进与培养计划项目(CIT&TCD20154043)