摘要
采用稀释涂布平板法,从食用玫瑰花瓣中分离到219株内生细菌。利用细菌菌落表征性状和16S rDNA序列对这些内生细菌进行了多样性分析。通过数值分析比较了8个菌落形态表征性状,以类平均连锁聚类法的方式进行聚类分析,在3.60的水平上可分成6个表观群。16S rDNA序列分析表明219株内生细菌可分为10个类群。经测序比对分析表明,这219株内生细菌分别与Gen Bank中3类细菌中的10个已知种相似性达到98.51%~100%。其中约氏不动杆菌（Acinetobacter johnsonii）、嗜麦寡养食单胞菌（Stenotrophomonas maltophilia）、Enhydrobacter aerosaccus是食用玫瑰花瓣可培养内生细菌的优势种群。
219 endophytic bacteria of Yunnan edible roses were isolated from petals by the method of grinding separation. A preliminary analysis was made on the diversity of the isolates by morphological characteristics and molecular classification methods based on partial 16 S rDNA sequences,and the analytical comparison was also made between them. 219 bacteria isolates were separated with 8 different morphological characteristics,and were grouped into 6 clusters at the level of 3. 60 with numerical classified based on the unweighted pair group method with arithmetic averages algorithm,and they were clustered into 10 molecular groups based on partial 16 S rDNA sequences. The result of sequence analysis of the 16 S rDNA showed the 98. 51 %- 100 % homology with 10 species in the Gen Bank. The dominant bacterial groups from petals of edible roses are Acinetobacter johnsonii,Stenotrophomonas maltophilia,Enhydrobacter aerosaccus.
出处
《西南农业学报》
CSCD
北大核心
2016年第6期1408-1413,共6页
Southwest China Journal of Agricultural Sciences
基金
云南省教育厅科学研究基金项目(2014Y390)
云南省应用基础研究青年项目(2013FD040)
昆明学院人才引进项目(YJL14005)
昆明学院人才引进基金项目(YJL12010)
昆明学院校立重点基金项目(XJL12020)
昆明学院大学生科研项目(XJD15054)
云南中烟品牌省内原料产地生态与主栽品种合理布局研究项目
云南省高校优势特色重点学科(生态学)建设项目资助(05000511311)
关键词
食用玫瑰
花瓣
可培养
内生细菌
微生物多样性
Edible roses
Flower petal
Culturablity
Endophytic bacteria
Diversity of microorganism
