摘要
为探讨鳃交换面积变化对鱼类静止代谢率的影响,采用剪除部分鳃弓处理了体质量为(47.6±1.0)g的鲫(Carassius auratus);实验设对照组、剪2个鳃弓组和剪4个鳃弓组,测定鲫剪鳃前后的代谢率、呼吸频率及鳃小片的相关参数。结果表明:剪鳃前,各组的静止代谢率、呼吸频率均无显著差异。剪鳃后,代谢率随时间延长先降低再升高,然后逐渐降低至剪鳃前水平;对照组呼吸频率处理时间点前后变化不显著,剪鳃弓组处理后呼吸频率随剪鳃弓数量的增多而显著增高。鳃小片密度及平均面积在各组间差异均不显著,剪鳃前、后也无显著变化。研究表明,剪鳃造成鲫的呼吸交换面积减小,该种鱼则可通过调节呼吸频率维持代谢需要而不依赖于鳃形态的可塑性;因此,在鱼类生理学实验操作中,通过剪鳃改变鳃呼吸面积是一种可行的方法。
To explore the effects of gill exchange area on resting metabolic rate of fish, the crucian carp （Carassius auratus） （47.6± 1.0） g were treated by shearing gill arch. The fish were sheared 0 （control group）, 2 and 4 branchial arches. The metabolic rate, ventilation frequency, and several parameters of branchial lamellae were determined before and after shearing, respectively. Resting metabolic rates did not differ among treatments before gill shearing. After gill shearing, the metabolic rate reduced rapidly, then rose up, and finally reduced gradually to the pre treated level. No significant difference in ventilation frequency was observed among groups prior to gill shearing. After gill shearing, ventilation frequency increased significantly with the shearing level. The ventilation frequency of the control group did not changed during the processing time. However, the ventilation frequency of the gill sheared fish increased significantly after shearing. The branchial lamellae density and average area were not significantly different among the three groups prior to gill shearing, and did not changed post shearing. Our results suggest that even the respiratory area of gill of the crucian carp was reduced by gill shearing, can keep the normal resting metabolic rate through physical adjustment after shearing the gills, its metabolic demand can be maintained by up-regulating ventilation frequency, rather than relying on the plasticity of the gill morphological structure. It also suggests that gill shearing can be used as a workable method to change the gill respiratory area of fish in future fish physiological study.
出处
《重庆师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第4期27-31,共5页
Journal of Chongqing Normal University:Natural Science
基金
国家自然科学基金(No.31000958)
重庆市自然科学基金(No.CSTC2013jcyjA80023)
重庆市食品药品检验检测研究院项目(No.2015092)
关键词
鲫
剪鳃
静止代谢率
Carassius auratus
shearing gills
resting metabolic rate
