摘要
本研究系统考察了阶梯降温并恢复至室温（33→25→20→15→10→22℃）长期变化过程（361d）厌氧氨氧化反应器的动态脱氮效能和厌氧氨氧化活性变化,分析了厌氧氨氧化污泥胞外聚合物（EPS）的变化特性,计算获得了厌氧氨氧化反应的活化能.结果表明,在温度20~33℃下,序批式厌氧氨氧化反应器可稳定高效运行,总氮去除负荷维持在0.4gN/（L·d）左右,最大比厌氧氨氧化活性（SAA）大于0.32gN/（gVSS·d）.10℃是厌氧氨氧化菌代谢活性的转折点：当温度降至10℃时,SAA 为0.044gN/（gVSS·d）,较33℃时下降91%.当温度恢复至22℃,厌氧氨氧化活性恢复至0.24gN/（gVSS·d）.厌氧氨氧化反应的活化能 Ea 在10~33℃和10~20℃范围内分别为68.4,152.9kJ/mol.在本实验温度（33→15℃）范围内,EPS 含量随着温度降低而升高；在10℃时,EPS 含量显著下降,出水中悬浮物升高,造成部分厌氧氨氧化污泥流失.
In this study, a long term dynamic change in anammox reaction in response to the gradient decreased and recovered temperature （33→25→20→15→10→22 ℃） was evaluated in a sequencing batch reactor （SBR） running over 360days; both the specific anammox activity （SAA） and extracellular polymers （EPS） contents were assessed. Results showed that anaerobic ammonia oxidation reaction in the SBR remained stable and efficient at 20~33℃; the total nitrogen removal load was 0.4gN/（L·d） and the SAA was over 0.32gN/（gVSS·d）. The temperature of 10℃ was the turning point for the anammox bacteria metabolic activity. At 10℃, anammox treatment capacity reduced significantly, with the SAA being decreased by 91% when compared with that at 33℃. However, anammox activity could be recovered to 0.24gN/（gVSS·d） at 22℃. The apparent activation energy （Ea） values was 68.4kJ/mol at 10~33℃ and 152.9kJ/mol at 10~20℃. In the range of 15~33℃, the EPS content of the anammox biomass increased with decreasing temperature;however, after temperature decreased to 10℃, the EPS content dropped significantly, and anammox sludge broke up into smaller flocs, leading to the loss of biomass from the bioreactor.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2016年第7期2006-2013,共8页
China Environmental Science
基金
国家自然科学基金资助项目(51522809
51378370)
国家重点实验室自主研究课题(PCRRY14001)
关键词
厌氧氨氧化
温度
活化能
胞外聚合物
SAA
anaerobic ammonium oxidation
temperature
apparent activation energy
specific anammox activity
extracellular polymer substance
