摘要
目的:探讨健脾益肺化痰方对慢性阻塞性肺疾病(COPD)气道黏液高分泌的改善机制。方法:采用烟熏法建立COPD大鼠模型,随机分为正常组、模型组、健脾益肺方组、化痰方组、健脾益肺化痰方组及羧甲司坦组。正常组、模型组灌胃生理盐水,各给药组灌胃相应药物,连续28 d。ELISA法检测肺泡灌洗液IL-13含量,WB法检测肺组织STAT6磷酸化水平,实时定量PCR法检测MUC5AC mRNA。结果:与正常组比较,模型组大鼠IL-13、p STAT6、MUC5AC表达增加(P<0.01);与模型组比较,各给药组IL-13、pSTAT6、MUC5AC表达下降(P<0.01)。结论:健脾益肺化痰方可通过抑制"IL13-pSTAT6-MUC5AC"信号通路改善COPD大鼠气道黏液高分泌,且"治本"作用优于"治标"。
Objective： To explore the mechanism of improvement of mucus hypersecretion in COPD rats by Jianpi Yifei Huatan Decoction. Methods： The rat model of COPD was established by tobacco smoke inhalation and then the rats were randomly divided into the normal group,model group,Jianpi Yifei Decoction group,Huatan Decoction group,Jianpi Yifei Huatan Decoction group and carbocisteine group. The normal group and model group were given normal saline solution intragastrically and other groups were given relevant medicines for 28 days,respectively. The expressions of IL-13,phospho-STAT6 and MUC5 ACmRNA in lung were detected by ELISA,western blot or RT-PCR,respectively. Results： Compared with the normal group,the expressions of IL-13,phospho-STAT6 and MUC5 AC mRNA in model group rats were significantly higher（ P 〈 0. 01）. Compared with model group,the expressions of IL-13,phospho-STAT6 and MUC5 ACmRNA in all treating groups’ rats were significantly lower（ P 〈 0. 01）. Conclusion： Jianpi Yifei Huatan Decoction improves airway mucus hypersecretion in COPD rats through inhibition of IL13-pSTAT6-MUC5 AC signaling pathway.
出处
《中华中医药学刊》
CAS
北大核心
2016年第8期1904-1907,共4页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81173314
81473666)
国家中医药管理局中医药重点学科基本科研项目(2013-ZDXKKF-03)
