摘要
建立高效液相色谱法测定腌渍蔬菜罐头中乙二胺四乙酸二钠含量的检测方法。试样中目标组分采用纯水提取,三氯化铁络合,PXA混合阴离子固相萃取柱净化,高效液相色谱检测,外标法定量。乙二胺四乙酸二钠的络合物质量浓度在1.0~100.0μg/m L范围内时,线性关系良好,其相关系数为0.999 95,检出限为0.09 mg/kg,定量限为0.31 mg/kg,添加量在0.03~0.3 g/kg范围内,平均加标回收率为99.09%~103.95%,相对标准偏差为0.4%~3.3%。该方法准确度高、重复性好,适合腌渍蔬菜罐头中乙二胺四乙酸二钠含量的检测。
A method for the determination of ethylenediamine tetraacetic acid disodium salt content in canned pickledvegetables was developed using high performance liquid chromatography （HPLC）. The target analyte was extracted withpure water. The extract was then derivatized with FeCl3, cleaned up by PXA mixed anion solid phase extraction, detectedwith HPLC detector and quantified by the external standard method. Under the optimal conditions, the calibration curveof ethylenediamine tetraacetic acid disodium salt derivatives was linear in the range of 1.0–100.0 μg/mL with correlationcoefficient of 0.999 95. The limit of detection （LOD） was 0.09 mg/kg and the limit of quantitation （LOQ） was 0.31 mg/kg.The average recoveries of spiked samples ranged from 99.09%–103.95%, with relative standard deviations （RSDs） between0.4% and 3.3% at spiked levels of 0.03–0.3 g/kg. The method is accurate, repeatable and suitable for the determination ofethylenediamine tetraacetic acid disodium salt in canned pickled vegetables.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第16期258-262,共5页
Food Science
基金
江西省教育厅科技项目(GJJ12417,GJJ11507)
国家卫生计生委办公厅食品安全国家标准项目(ZHENGHE-2014-287)
关键词
乙二胺四乙酸二钠
腌渍蔬菜罐头
高效液相色谱法
固相萃取
ethylenediamine tetraacetic acid disodium salt
canned pickled vegetables
high performance liquidchromatography （HPLC）
solid phase extraction
