结核诊断中应用T细胞斑点试验的效果分析被引量：6

Effect of T cell spot test on TB diagnosis

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摘要目的分析结核分枝杆菌T细胞斑点试验(T-SPOT.TB)技术在结核性胸膜炎诊断中的诊断效果,为临床诊断提供参考依据。方法选取2012年5月-2015年5月在医院就诊的结核性胸膜炎患者192例为观察组,选取同期非结核性胸腔积液患者190例为对照组,对结核分枝杆菌早期分泌靶抗原6(ESAT-6)和(或)培养虑液蛋白10(CFP-10)致敏的T细胞,并与PPD皮内试验、腺苷脱氢酶(ADA)、胸水结核分枝杆菌聚合酶链反应(TB-DNA)进行比较,数据采用SPSS 19.0软件进行统计分析。结果观察组患者ESAT-6、CFP-10、E/C阳性率分别为85.4%、75.0%,91.7%,均高于对照组,差异均有统计学意义(P<0.05);T-SPOT.TB(E/C)敏感度、约登指数、诊断准确率明显高于T-SPOT.TB(ESAT-6)和T-SPOT.TB(CFP-10);T-SPOT.TB(E/C)诊断敏感度、特异度、约登指数及诊断准确率优于其他方法,经统计检验,差异有统计学意义(P<0.05)。结论结核分枝杆菌T细胞斑点试验技术在结核性胸膜炎诊断敏感度、特异度和准确率较高。 OBJECTIVE To analyze the T cell spot test of Mycobacterium tuberculosis（T-SPOT.TB）technique in the diagnosis of tuberculous pleurisy,so as to provide references for clinical diagnosis.METHODS A total of 192 cases of tuberculous pleurisy patients in hospital from May 2012 to May 2015 were selected as observation group,another 190 cases of non-tuberculous pleural effusion at the same period were selected as control group.M.tuberculosis early secretory antigenic target 6（ESAT-6）and/or culture filtrate protein 10（CFP-10）sensitized T-cells,and the PPD skin test,adenosine dehydrogenase（ADA）,pleural effusion by polymerase chain reaction（TBDNA）were compared.RESULTS The positive rates of ESAT-6,CFP-10,amd E/C of observation group were85.4%,75.0%,and 91.7%,which were significantly higher than those of control group（P〈0.05）.T-SPOT.TB（E/C）sensitivity,Youden index and diagnostic accuracy rate were significantly higher than T-SPOT.TB（ESAT-6）and T-SPOT.TB（CFP-10）.T-SPOT.TB（E/C）diagnostic sensitivity,specificity,Youden index and diagnostic accuracy rate were superior to other methods,and the differences were significant（P〈0.05）.CONCLUSION M.tuberculosis T cell spot test technique has high sensitivity,specificity and accuracy rate in the diagnosis of tuberculous pleurisy,and it has references for clinical.

作者李秀萍王玉清王玲程国栋韩连存

机构地区青海省第四人民医院呼吸一科青海省第四人民医院检验科

出处《中华医院感染学杂志》 CAS CSCD 北大核心 2016年第16期3645-3647,共3页 Chinese Journal of Nosocomiology

基金青海省卫生厅基金资助项目(QW-2010B043)

关键词结核感染 T细胞斑点试验结核诊断 Tuberculosis infection T cell spot test Tuberculosis Diagnosis

分类号 R446 [医药卫生—诊断学]

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参考文献6

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