摘要
以6个代表性大豆品种制备11S球蛋白,研究大豆11S球蛋白结构特性与表面疏水性关系。采用ANS荧光探针法测定表面疏水性,Ellman试剂分析法测定巯基和二硫键含量,激光拉曼光谱和荧光光谱分析空间构象。结论表明,大豆11S球蛋白表面疏水性与α-螺旋含量、β-折叠含量负相关,与β-转角含量、无规则卷曲含量正相关;与拉曼光谱色氨酸费米共振I1360/I1340值负相关,与拉曼光谱酪氨酸费米共振I850/I830值正相关,与暴露酪氨酸残基克分子数正相关,与N暴露N包埋值正相关;与暴露巯基含量、巯基暴露程度正相关,与游离巯基含量、二硫键含量、二硫键构象相关性不显著。
In this study, globulin （11S） was prepared with soybean from six representative regions as raw material, and the relationship between the structure characteristics and surface hydrophobicity of 11S was studied. The surface hydrophobicity of 1 1S was determined by ANS fluorescent probe method, the content of sulfhydryl and disulfide bond was determined by EIIman reagent analysis method, and con- formation was analyzed by Raman spectroscopy and fluorescence spectroscopy. The main research results were as follows： correlation analysis showed that there was significantly negative correlation between surface hydrephobicity and the content of a-helix, β-sheet and Fermi resonance Raman spectra of tryptophan I136/I1340 ratio. There was positive correlation between surface hydrophobicity and β-turn, random coil, Fermi resonance Raman spectra of tryptophan I850/I830 ratio, the number of exposed tyrosine residues, the value of Nexposure： Nembedding and the content of exposed sulfhydryl. There was not correlation between the surface hydrophobicity and the content of free sulfhydryl, the content of disulfide bond and disulfide bond conformation.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2016年第7期32-39,共8页
Journal of Northeast Agricultural University
基金
国家科技支撑计划项目(2014BAD22B01)
国家自然科学基金面上项目(31071493)
宁德师范学院服务闽东项目(2012H314)
宁德师范学院6.18项目(2012H213)
关键词
大豆11S球蛋白
拉曼光谱
荧光光谱
巯基和二硫键
表面疏水性
soybean globulin（11S）
raman spectrum
fluorescence spectrum
sulfhydryl and disulfide bond
surface hydrophobicity
