摘要
目的了解广州市乙型肝炎病毒表面抗原（HBs Ag）阳性母亲所生新生儿乙肝免疫球蛋白（HBIG）及疫苗接种的应答水平及乙肝病毒变异情况。方法从广州市黄埔区、荔湾区、从化市、番禺区、天河区、越秀区6个区助产医院中,选取HBs Ag阳性产妇及其所分娩的接种乙肝免疫球蛋白及疫苗的新生儿,出生后接种HBIG及乙肝疫苗（3针）,检测HBs Ag、抗乙型肝炎表面抗原抗体（抗-HBs）及乙肝病毒脱氧核糖核酸（HBV DNA）。结果共调查新生儿11 831名,HBs Ag筛查率为100.00%,其中HBs Ag阳性10名,抗-HBs阳性11 239名,HBs Ag携带率0.08%（10/11 831）;对HBs Ag阴性但乙肝HBV DNA阳性者进行S基因测序,流行的乙肝病毒基因型有2种,其中B基因型70.00%、C基因型30.00%,不同毒株间核苷酸同源性为93.15%~99.91%;抗-HBs无（弱）应答者采取复种免疫后,抗-HBs阳转率达90.00%~92.00%,GMT（几何平均滴度）在260.02~304.37 m IU/ml间。结论广州市HBs Ag阳性母亲所生新生儿HBIG及疫苗接种效果明显;S基因序列和各参考株高度同源,未发现其他基因型及有生物学意义的变异;对无（弱）应答者采取复种免疫,抗-HBs阳转率显著提高。
Objective To understand the response level of Hepatitis B immunoglobulin （HBIG） and Hepatitis B virus （HBV） and Hepatitis B virus （HBV） variation in the Hepatitis B virus surface antigen （HBsAg） positive mothers in Guangzhou. Methods From the Huangpu district, Liwan district, conghua, panyu district, tianhe district, yuexiu district six district midwifery hospital in Guangzhou select the Hepatitis B virus surface antigen （HBsAg） positive maternal and the delivery of newborns vaccinated against hepatitis b immune globulin and, after the birth of HBIG and hepatitis b vaccine inoculation needle （3 pin）, detection of HBsAg, anti Hepatitis B surface antigen antibody （anti - HBs） and Hepatitis B virus DNA （DNA HBV） . Results A total of 11 831 survey of neonatal, HBsAg screening rate was 100. 00% , among them 10 HBsAg positive, anti - HBs positive number is 11 239, positive HBsAg carry rate was 0. 08% （ 10/11 831 ） ; The HBV DNA positive for HBsAg negative but hepatitis b S gene sequencing, two dominant HBV genotypes were identified, genotypes B and C （70. 00% and 30. 00% respectively） . Different HBV strains share the nucleotide homology of the S gene between 93.15% and 99.91%. Anti - HBs no （weak） respondents take revaccination after immunization, Anti - HBs positive rate of 90. 00% - 92.00% , GMT （ geometric mean titer） in 260. 02 - 304. 37 mIU/ml. Conclusion The effect of HBIG and vaccination of the newborn HBsAg in the mother of Guangzhou was obvious; The S gene sequence analysis of the prevalent HBV strains in Guangzhou showed the reference strains, and only two major HBV genotypes （ B and C） were found. For no （weak） respondents take revaccination immune, anti - HBs positive rate increased significantly.
出处
《医学动物防制》
2016年第7期735-738,共4页
Journal of Medical Pest Control
基金
广东省医学科研基金立项课题(A2014588)
