摘要
目的:探讨γδT细胞对卵巢癌SKOV3细胞增殖的抑制作用,阐明γδT细胞诱导卵巢癌细胞凋亡的可能作用机制。方法:将体外培养的人卵巢癌SKOV3细胞设为对照组,将体外分离培养且与γδT细胞共培养72h的卵巢癌SKOV3细胞设为γδT细胞处理组,于激光共聚焦显微镜下观察2组中SKOV3细胞核形态学变化,采用MTT法检测2组SKOV3细胞增殖抑制率,利用Transwell小室检测细胞迁移能力,采用流式细胞术检测细胞凋亡率。结果:激光共聚焦显微镜下,γδT细胞处理组SKOV3细胞出现核皱缩等细胞凋亡的形态学改变较对照组明显;MTT法检测,γδT细胞处理组SKOV3细胞增殖抑制率高于对照组(P<0.05);Transwell小室检测,γδT细胞处理组SKOV3细胞穿膜细胞数少于对照组,细胞的迁移率低于对照组(P<0.05);流式细胞术检测,γδT细胞处理组SKOV3细胞凋亡率高于对照组(P<0.05)。结论:γδT细胞能够抑制卵巢癌SKOV3细胞的增殖及迁移能力,促进细胞凋亡。
Objective：To study the inhibitory effect of γδT cells on the proliferation of ovarian cancer SKOV3 cells,and to clarify its possible mechanism of inducing apoptosis. Methods：The human ovarian cancer SKOV3 cells cultured in vitro were used as control group,and theγδT and SKOV3 cells were co-cultured for 72 h as γδT cells treatment group.Laser scanning confocal microscope was used to obeserve the morphological changes of nucleus SKOV3 cells,and the inhibitory rate of proliferation of SKOV3 cells in two groups were detected by MTT method;Transwell Chambers was used to detect the cell migration ability,then the apoptotic rates of SKOV3 cells were tested by flow cytometry （FCM）.Results：The apoptotic morphology of nucleus of SKOV3 cells in γδT cells treatment group were found under microscope,such as nuclear shrinkage.The MTT resultes displayed that the inhibitory rate of proliferation of SKOV3 cells in γδT cells treatment group was higher than that in control group （P 〈0.05）.The Transwell Chambers results showed that the number of transmembrane cells in γδT cells treatment group was lower than that in control group,and the migration rate was decreased compared with control group （P 〈0.05）.The FCM results showed that the apoptotic rate of SKOV3 cells in γδT cells treatment group was higher than that in control group （P 〈 0.05 ）.Conclusion：γδT cells can inhibit the proliferation and the migration abilities of ovarian cancer SKOV3 cells,and promote the apoptosis.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2016年第5期897-900,I0002,共5页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科技发展计划项目资助课题(20120718)
