摘要
旨在研究TLR家族基因在产蛋高峰期和停产期鸭肠道中的表达。选择同日龄产蛋高峰期鸭20只,随机均分为两组,一组在限食7d停产后自由采食10d,另一组自由采食17d。采用RT-PCR和qRT-PCR的方法,检测TLR1-1、TLR1-2、TLR2-1、TLR2-2、TLR3、TLR4、TLR5、TLR7、TLR15和TLR21基因在鸭十二指肠、空肠、回肠和盲肠中的表达。结果显示,10种禽类TLR家族基因均在鸭十二指肠、空肠、回肠和盲肠中表达;除TLR1-2、TLR2-2、TLR4和TLR21基因外,其他6种TLR家族基因在不同肠道中差异表达(P<0.05),其中,TLR3、TLR5和TLR7基因在十二指肠中高表达,TLR1-1、TLR2-1和TLR15基因在盲肠中高表达;除TLR4和TLR7基因外,其他8种TLR家族基因在产蛋高峰期鸭肠道中的表达水平高于停产期(P<0.05)。结果表明,蛋鸭肠道存在TLR介导的固有免疫反应机制,且产蛋高峰期蛋鸭肠道病原相关分子模式的识别能力强于停产期蛋鸭。
This study aimed to investigate expression of toll-like receptors in different segments of intestinal tract in duck at the peak and end of lay.Twenty laying ducks at the peak of lay were randomly assigned to 2groups.One group ducks were given a restricted feed for 7days,and then provided with feed ad libitumfor 10 days.Another group ducks were provided with feed ad libitumfor 17 days.Expression of TLR1-1,TLR1-2,TLR2-1,TLR2-2,TLR3,TLR4,TLR5,TLR7,TLR15 and TLR21were analyzed in duck duodenum,jejunum,ileum and caecum by RT-PCR and qRTPCR methods.The results showed that 10 TLRs genes were all expressed in duodenum,jejunum,ileum and caecum.Except for TLR1-2,TLR2-2,TLR4 and TLR21,the other TLRs genes mRNA expression was significantly different among different segments of duck intestinal tract（P〈0.05）.Of these,TLR3,TLR5 and TLR7mRNA expression were higher in duodenum than in the other tissues,while TLR1-1,TLR2-1and TLR15 mRNA expression were higher in caecum than in the other tissues.Except for TLR4 and TLR7,the other TLRs genes mRNA expression was higher in ducks at the peak of lay than that in ducks at the end of lay（P〈0.05）.In conclusion,a TLR-mediated innate immune response mechanismexists in the laying duck intestinal tract,and there was stronger ability to recognize pathogens in duck intestinal tract at the peak of lay than that at the end of lay.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2016年第10期2012-2019,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
江苏省农业科技自主创新资金(CX(12)3070)
国家水禽产业技术体系(CARS-43-16)
关键词
蛋鸭
TOLL样受体
肠道
产蛋高峰期
停产期
laying duck
toll-like receptors（TLRs）
intestinal tract
peak of lay
end of lay
