摘要
目的:探讨子痫前期(PE)患者外周血、新生儿脐血血清和胎盘组织中转化生长因子β_1(TGF-β_1)的表达及其临床意义。方法:采用病例-对照研究方法,对2015年1月1日至2015年12月31日在本院产检并分娩的正常妊娠组(对照组,n=50)和PE组(n=54,其中早发型PE组29例,晚发型PE组25例)使用酶联免疫吸附法及蛋白印迹法分别检测两组孕妇外周血、新生儿脐血血清及胎盘组织中TGF-β_1表达水平,分析与临床指标的关系。结果:①两组在入组年龄、分娩孕周、定期产检、最高收缩压、舒张压、新生儿体质量、血肌酐、尿酸、白蛋白方面差异有统计学意义(P<0.01)。②PE组孕妇外周血血清中TGF-β_1水平与年龄负相关(r=-0.289,P<0.05),与发病孕周、最高血压、新生儿体质量、白蛋白、血肌酐、尿酸无明显相关(P>0.05)。③早发型P E组和晚发型PE组孕妇外周血血清TGF-β_1水平显著高于对照组(P<0.05),但新生儿脐血血清TGF-β_1水平比较差异无统计学意义(P>0.05)。④早发型PE组和晚发型PE组孕妇胎盘组织中TGF-β_1的表达水平显著高于对照组(P<0.05),早发型PE组、晚发型PE组TGF-β_1蛋白表达水平比较差异无统计学意义(P>0.05)。结论:TGF-β_1可能参与PE的病理生理和发病机制,随着年龄的增长,其在母体外周血血清表达越低。孕晚期检测母体外周血血清TGF-β_1水平,可能有助于高危人群的筛查。
Objective：To investigate the different expressions of TGF-β1 in serum and placenta between normal pregnant and pregnant with preeclampsia,and to explore its clinical significance forpreeclampsia. Methods：This study is case-control study that included 104 women came from the department of Obstestric of the Third Affiliated Hospital of GuangZhou Medical University from 2015-1 -1 to 2015-12-31 .50 normal pregnant （ control group） and 54 preeclamptic women（ preeclampsia group, divided into two subgroup ：29 in early on- set preeclampsia group,25 in late onset preeclampsia group ）. The levels of TGF-β1 in serum and placenta were detected by enzyme linked immunosorbent assay and western blot respectively, and their relations with the clinical indexes were also analyzed. Results： ①There was significant statistical difference on included age, gestational age, the antenatal care, the highest systolic blood pressure, diastolic blood pressure, new- born body weight, albumin, serum creatinine anduric acid between the two groups（ P 〈 0.01 ）. ②The level of TGF-β1 in serum from peripheral blood in the preeclampsia group was negatively related to the age （r = - 0. 289, P 〈 0.05）, and was not statistically related to the onset gestational age, the highest systolic blood pressure, newborn body weight, albumin, serum creatinine and anduric acid （ P 〉 0.05 ）. ③The level of TGF-β1 in serum from peripheral blood of the preeclampsia group were significantly higher than that of control group（ P〈0. 05）. There was no significant difference on the level of TGF-β1 in serum from neonatal umbilical cord blood between the two groups（ P〉0.05）. ④The level of TGF-β1 in placenta of the preeclampsia group（ including early onset preeclampsia group and late onset preeclampsia group） were significantly higher than that of control group（ P 〈0.05）,there was no difference on the expression of TGF-β1 protein between the early onset preeclampsia group and late onset preeclampsia group （ P 〉 0.05）. Conclusions ： TGF-β1 May participate in the occurrence and development of preeclampsia, and has an negative correlation with age, detection of the TGF-β1 level in the peripheral blood of the late pregnancy women is recommended which could help in the screening of the high risk population.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2016年第10期751-755,共5页
Journal of Practical Obstetrics and Gynecology
基金
广东省医学科学技术研究基金项目(编号:A2016182)
