摘要
[目的]探讨尘肺患者周围血1,25-二羟维生素D_3[1,25-(OH)_2D_3]水平和肺纤维化相关细胞因子水平以及它们之间的相互关系。[方法]选取某矿务集团职业病防治院2015年4—5月期间就诊的男性尘肺患者43人为观察组,其中尘肺壹期28人,尘肺贰期15人;选取同期27名男性未接尘健康体检者为对照组。收集两组对象的一般信息,采集晨起空腹周围血制备血清,采用电化学发光法检测周围血1,25-(OH)_2D_3水平,采用双抗夹心ELISA法检测两组周围血转化生长因子β1(TGF-β1)、白介素4(IL-4)、γ干扰素(INF-γ)水平。采用单因素方差分析检验其组间差异,多组间两两比较则采用SNK检验;相关性检验采用Pearson相关分析。[结果]对照组周围血1,25-(OH)_2D_3水平为(22.16±5.71)μg/L,尘肺壹期组为(13.11±4.01)μg/L,尘肺贰期组为(9.96±3.18)μg/L;与对照组相比,尘肺患者周围血1,25-(OH)_2D_3水平降低,且随着尘肺期别升高而降低(P<0.01)。对照组周围血TGF-β1水平为(25.59±6.12)μg/L,尘肺壹期组为(39.20±6.32)μg/L,贰期组(50.90±5.97)μg/L;与对照组相比,尘肺患者周围血TGF-β1水平升高,且随着尘肺期别升高而升高(P<0.01)。对照组周围血IL-4水平为(25.03±3.79)ng/L,尘肺壹期组为(32.67±5.32)ng/L,尘肺贰期组为(37.52±5.71)ng/L;与对照组相比,尘肺患者周围血IL-4水平随着尘肺期别升高而升高(P<0.01)。对照组周围血INF-γ水平为(32.53±6.50)ng/L,尘肺壹期组为(16.64±4.06)ng/L,尘肺贰期组(13.70±2.98)ng/L;与对照组相比,尘肺患者周围血INF-γ水平降低(P<0.01);但尘肺壹期与贰期之间的差异无统计学意义(P>0.05)。尘肺患者周围血1,25-(OH)_2D_3水平与INF-γ水平呈正相关(r=0.944,P<0.05),与TGF-β1及IL-4水平呈负相关(r分别为-0.814,-0.937,均P<0.05)。[结论]尘肺患者周围血1,25-(OH)2D_3水平与肺纤维化相关细胞因子的表达水平相关联,可能与尘肺发病相关。
[Objective ] To measure the levels of 1, 25-dihydroxy vitamin D3 [1, 25-（OH）2D3] and pulmonary fibrosis related cytokines in peripheral blood of pneumoconiosis patients, and assess the correlation between them. [ Methods ] From April to May 2015, 43 male patients visiting an occupational disease prevention and treatment center affiliated to a mining corporation were selected as the pneumoconiosis group （28 cases diagnosed as stage I and 15 cases as stage Ⅱ）. In the same period, 27 males without dust exposure history ordered health check services were selected as the control group. Their general information and fasting blood samples were collected. Electrochemiluminescence was used to detect 1, 25-（OH）2D3 level and ELISA was used to detect transforming growth factor Ⅲ （TGF-β1）, interleukin-4 （IL-4）, and T-interferon （INF-T） levels. The differences between indicators were evaluated through one-way analysis of variance （ANOVA） followed by pair-wise comparison using Student-Newman-Keuls test. Pearson correlation analysis was also applied. [ Results ] The 1, 25-（OH）2D3 level of the control group was （22.16± 5.71）μg/L, that of the pneumoconiosis patients in stage I was （13.11±4.01）μg/L, and that of the pneumoconiosis patients in stage I] was （9.96±3.18） μg/L; compared with the control group, the 1, 25-（OH）2D3 level of the pneumoconiosis patients was reduced with the advance of pneumoconiosis stage （P〈 0.01）. The TGF-β1 level of the control group was （25.59±6.12）μg/L, that of the pneumoconiosis group in stage I was （39.20±6.32） μg/L, and that of pneumoconiosis group in stage [I was （50.90±5.97）μg/L; compared with the control group, the TGF-β1 level of the pneumoconiosis patients was elevated with the advance of pneumoconiosis stage （P〈 0.01）. The IL-4 level of the control group was （25.03±3.79） ng/L, that of the pneumoconiosis group in stage I was （32.67±5.32） ng/L, and that of the pneumoconiosis group in stage I1 was （37.52 ±5.71） ng/L; compared with the control group, the 1L-4 level of the pneumoconiosis patients was elevated with the advance of pneumoconiosis stage （P〈0.01）. The INF-7 level of the control group was （32.53± 6.50） ng/L, that of pneumoconiosis group in stage I was （16.64±4.06） ng/L, and that of the pneumoeoniosis group in stage Ⅱ was （13.70± 2.98） ng/L; compared with the control group, the INF-γ level of the pneumoconiosis patients was decreased （P 〈 0.01）, but there was no significant difference between the pneumoeoniosis patients in stage I and stage II （P〉0.05）. The peripheral 1, 25-（OH）2D3 levels of the pneumoeoniosis patients had a positive correlation with INF-y levels （r=0.944, P〈0.05）, while a negative correlation with TGF-131 andlL-41evels（r=-0.814, r=-0.937, bothPs〈0.05）. [Conclusion] The peripheral 1,25-（OH）2D31evelofpneumoconiosispatients associates with the expression levels of pulmonary fibrosis related cytokines and might involve in the pathogenesis of pneumoeoniosis.
出处
《环境与职业医学》
CAS
CSCD
北大核心
2016年第10期977-980,共4页
Journal of Environmental and Occupational Medicine
基金
徐州市科技局项目(编号:KC14SH066)
徐州矿务集团总医院科研项目(编号:Y2015013)
