摘要
目的探讨细胞蜡块联合免疫组化方法在恶性浆膜腔积液诊断中的应用价值。方法对87例经证实的恶性浆膜腔积液病例,采用常规细胞学涂片与细胞块切片结合免疫组化检测研究。结果87例恶性浆膜腔积液的细胞类型及来源进行分类,胸水52例其中腺癌48例,35例来源于肺,8例来源于卵巢,3例来源于乳腺,2例来源于胃肠道;T淋巴母细胞性淋巴瘤1例;恶性黑色素瘤1例;鳞癌1例,恶性间皮瘤1例。腹水35例,其中腺癌34例,20例来源于卵巢,13例来源于胃肠道,1例来源肺,弥漫大B细胞淋巴瘤1例。常规细胞学涂片+细胞块联合免疫组化检出率明显优于常规细胞学涂片(P<0.001)。结论细胞块联合免疫组化技术在恶性浆膜腔积液诊断中具有重要的临床意义。
Objective To explore the application value of cell block combined with immunohistochemistry in diagnosis of malignant serous cavity effusion. Methods Malignant serous cavity effusion of 87 cases were confirmed, and conventional cytological smears,cell block slice combined with immunohistochemical detection were used.Results Among 87 cases of serous cavity effusion,there were 52 cases belonging to hydrothorax. And among 52 hydrothorax cases,there were 48 cases caused by adenocarcinoma,including 35 cases came from the lung,8 cases came from the ovary,3 cases came from the breast,2 cases came from the gastrointestinal tract,1 case caused by T- lymphoblastic lymphoma,1 case caused by malignant melanoma,1 case caused by squamous cell carcinoma,1 case caused by malignant mesothelioma. There were 35 cases belonging to ascites,in which 34 cases were caused by adenocarcinoma,including 20 cases came from the ovary,13 cases came from the gastrointestinal tract,1 case came from the lung,1 case caused by diffuse large B cell lymphoma. Detection rate of conventional cytological smears plus Cell block combined with immunohistochemistry was superior to conventional cytological smears（ P 〈0. 001）. Conclusions Cell block and immunohistochemical technique combination in the diagnosis of serous cavity effusion has important clinical significance.
出处
《齐齐哈尔医学院学报》
2016年第23期2873-2875,共3页
Journal of Qiqihar Medical University
关键词
恶性浆膜腔积液
细胞块
免疫组化
Malignant serous cavity effusion
Cell block
Immunohistochemistry
