摘要
目的：建立HPLC波长切换联合梯度洗脱法测定补肾益精丸中的异去甲基蟛蜞菊内酯、蟛蜞菊内酯、金丝桃苷、紫云英苷和异鼠李素。方法：采用kromasilC18（250mm×4．6mm，5μm）色谱柱；流动相：乙腈（A）和0．4％磷酸溶液（B），进行梯度洗脱；流速：0．9mL／min；柱温：30℃；进样量为20汕。异去甲基蟛蜞菊内酯和蟛蜞菊内酯的检测波长为351nm，金丝桃苷、紫云英苷和畀鼠李素的检测波长为360nm。结果：异去甲基蟛蜞菊内酯、蟛蜞菊内酯、金丝桃苷、紫云英苷和异鼠李素分别在5．15-103．00μg/mL（r=0．9992）、4．58-91．60μg／mL（r=0．9998）、9．95-199．00μg／mL（r=0．9996）、8．27-165．40μg／mL（r=0．9999）、4．55-91．00μg／mL（r=0．9994）范围内与峰面积具有较好的线性关系，平均加样回收率和相应的RSD（n=6）分别为97．59％（1．06％）、99．33％（1．17％）、98．18％（0．92％）、98．95％（1．11％）、97．05％（0．77％）。结论：本文建立的HPLC波长切换梯度洗脱法同时测定补肾益精丸中的5个成分：异去甲基蟛蜞菊内酯、蟛蜞菊内酯、金丝桃苷、紫云英苷和异鼠李素，方法操作简便、快速，可作为补肾益精丸全面可靠的质量控制方法。
Objective：To develop an HPLC wavelength switching gradient elution method for contents determination of Demethylwedelolactone,Wedelolactone, Hyperoside, Astragaline and Isorhamnetin in Bushen Yijing Pill simultaneously. Method：A kromasil C18 （250mm×4.6mm,5μm） column was used as the chromatographic column. A mobile phase of acetonitrile（A） and 0.4% phosphate acid（B） as the mobile phase were adopted. The flow rate was 0.9 mL/min and the column temperature was set at 30 ℃. The sample quantity was 20μL. Demethylwedelolactone and Wedelolactone were detected at 351 nm. Hyperoside,Astragaline and Isorhamnetin were detected at 360 nm. Results：There was a good linear relationship between the concentrations of Demethylwedelolactone, Wedelolactone, Hyperoside, Astragaline and Isorhamne- tin peak area value when the concentrations of Demethylwedelolactone, Wedelolactone, Hyperoside, Astragaline and Isorh- amnetin were within the range of 5.15 - 103.00 μg/mL（ r = 0. 999 2） ,4.58 - 91.60 μg/mL（ r = 0. 999 8 ） ,9.95 199. 00 μg/mL（r =0.999 6） ,8.27 - 165.40 μg/mL（r =0. 999 9） ,4.55 - 91.00 μg/mL（r =0. 999 4）. The average recoveries were 97.59% （ 1.06% ） ,99.33% （ 1.17% ） ,98.18% （0.92%） ,98.95% （ 1.11% ） and 97.05% （0.77%） , respectively. Conclusion： An HPLC wavelength switching combined gradient elution method has been established for sim- uhaneous determination of 5 components in Bushen Yijing Pill. The method is simple, quick and helpful for the quality control of Bushen Yijing Pill.
出处
《中华中医药学刊》
CAS
北大核心
2016年第11期2798-2801,共4页
Chinese Archives of Traditional Chinese Medicine
关键词
HPLC
波长切换法
补肾益精丸
异去甲基蟛蜞菊内酯
蟛蜞菊内酯
金丝桃苷
紫云英苷
异鼠李素
HPLC
wavelength switching combined gradient elution method
Bushen Yijing Pill
Demethylwedelolac-tone
Wedelolactone
Hyperoside
Astragaline
Isorhamnetin
