摘要
【目的】研究印度菜粕替代国产菜粕对奥尼罗非鱼生长性能、免疫力及肝脏结构的影响。【方法】选取120尾初始体质量约为17.3 g的奥尼罗非鱼,随机分为4组;设计4种等氮等能的实用饲料G0、G50、G75和G100,其中,G0为对照组（国产菜粕用量35%）,G50、G75和G100为分别用印度菜粕替代G0饲料中50%、75%和100%的国产菜粕并添加质量分数为0.1%的二甲基-β-丙酸噻亭（DMPT）为诱食剂,饲养周期为43 d。【结果】印度菜粕替代水平升高可导致罗非鱼的末均质量、增质量率、特定生长率降低,饲料系数升高,其中,G100组差异显著（P〈0.05）。全鱼体成分和血清常规生化指标差异不显著（P〉0.05）,血清丙二醛（MDA）、NO含量和超氧化物歧化酶（SOD）、碱性磷酸酶（AKP）活性差异也不显著（P〉0.05）,但G100组总抗氧化能力（T-AOC）和溶菌酶（LZM）含量比G0显著升高（P〈0.05）。随着印度菜粕替代水平的升高,奥尼罗非鱼肝细胞结构的损伤程度逐渐加大。【结论】生长阶段在17~70 g的奥尼罗非鱼,饲料中印度菜粕替代国产菜粕的水平不宜超过75%。
[ Objective ] To evaluate the effects of China rapeseed meal replaced by Indian rapeseed meal on growth performance, immune and liver function of juvenile tilapia, Oreochromis niloticus x O. aureus. [ Method] A total of 120 fish with about 17.3 g an initial body mass per fish were randomly divided into four groups. A feeding trial with four practical diets at equal nitrogen and caloric basis was conducted. Group GO was the control group （the amount of China rapeseed meal was 35% ）, and G50, G75 and G100 were trial groups, for which Indian rapeseed meal replaced 50% , 75% and 100% of the China rapeseed meal in GO, respectively, and 0. 1% feed attraetant DMPT was added. The feeding cycle was 43 d. [ Result ] With the percentage of India rapeseed meal increasing, the final mass, mass gain rate and specific growth rate of tilapia decreased while the feed conversion ratio increased, with significant differ- ences only detected for the G100 group （P 〈 0. 05） compared to the GO group. There was no significant difference （P 〉 0.05 ） in body composition, serum biochemical indices, serum malondialdehyde （MDA） contents, nitric oxide （NO） contents, superoxide dismutase （SOD） activity or alkaline phosphatase （AKP） activity among different groups （ P 〉 0.05 ）. However, the total antioxidant capacity （ T-AOC ） and lysozyme （LZM） contents of the G100 group were significantly higher than those of the GO group （ P 〈 0. 05）. The liver cell structure of tilapia was damaged more seriously with the percentage of Indian rapeseed meal increasing. [ Conclusion] The percentage of India rapeseed meal replacing China rapeseed meal should not exceed 75% for tilapia, O. niloticus x 0. aureus with 17 -70 g body mass.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2016年第3期9-16,共8页
Journal of South China Agricultural University
基金
国家科技型中小企业创新基金(12C26214405244)
广东省科技型中小企业创新基金(2012CY002)
关键词
奥尼罗非鱼
印度菜粕
生长性能
免疫
肝功能
Oreochromis niloticus x O. aureus
India rapeseed meal
growth performance
immune
liver function
