摘要
土壤中分离筛选到一株对柴油具有降解作用的菌株,通过菌落形态观察及生理生化实验,将该菌株归属为假单胞菌属(Pseudomonas)。在单因素实验的基础上,采用响应面法设计,对菌株降解柴油的降解条件(初始p H、SDS添加量和培养时间)进行优化,建立了菌株对柴油的降解率随培养基的初始p H、SDS添加量和培养时间的多项二次回归方程,并得到最适的降解条件是:初始p H为6.5,SDS添加量为109mg/L,培养时间为6.7d时,菌株对柴油的降解率可达89.53%,比优化前提高了0.4倍,预测模型可靠,可利用该模型对菌株降解柴油的最优降解条件进行理论预测。
A strain with diesel to Pseudomonas was identified oil degradation capacity was isolated from the soil. The strain which belongs by colony morphology as well as physiological and biochemical experiments. Based on the single factor experiment, the fermentation conditions （initial pH, SDS dosage and culture time） of the strain were optimized by response surface analysis, and a quadratic regression equation with the variables of initial pH, SDS dosage and culture time was established. The results showed that optimal conditions were initial pH = 6.5, SDS=109 mg/l, culture time = 6.7d; Under optimized conditions, the degradation rate of the strain was as high as 89.53% , an 0.4 times increase over pre-optimization. The prediction model is highly reliable and can be used to predict optimal conditions for the strain＇s degradation of diesel oil.
出处
《福建师大福清分校学报》
2016年第5期42-48,共7页
Journal of Fuqing Branch of Fujian Normal University
基金
福建省教育厅中青年教师项目(JA15569)
福建省自然科学基金面上项目(2015J01132)
关键词
假单胞菌属
柴油降解菌
降解率
响应面分析
pseudomonas
Diesel degrading bacteria
degradation rate
response surface analysis
