摘要
目的:观察芹菜素纳米乳对CCl4诱导大鼠肝纤维化模型的作用及对基质金属蛋白酶-1(MMP-1)、金属蛋白酶抑制因子-1(TIMP-1)的影响。方法:采用CCl4复制大鼠肝纤维化模型,自造模之日开始,灌胃给药,1次/d,连续8周。末次给药后24 h,取大鼠血和肝脏,观察芹菜素纳米乳对血清肝功能指标(ALT、AST)、肝纤维化指标(HA、LN、Ⅲ-C、Ⅳ-C)和肝组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、羟脯氨酸(Hyp)、MMP-1、TIMP-1水平的影响。结果:与模型组比较,各给药组肝功能和肝纤维化均有不同程度的改善。结论:芹菜素纳米乳对CCl4诱导肝纤维化大鼠具有一定的治疗作用,其作用可能与抗脂质过氧化反应、影响MMP-1和TIMP-1表达有关。
AIM： To observe therapeutic effects of apigeninnanoemulsion on CCl4 induced hepatic fibrosis and hepatic tissue level of MMP-1,TIMP-1 in rats. METHODS： The model of rat liver fibrosis was adopted which was induced by CCl4 injection.Drugs were administrated from the first day for 8weeks,once a day. 24 hours after the last administration,the levels of hepatic function indices（ ALT,AST） and hepatic fibrosis indices（ HA,LN,Ⅲ-C,Ⅳ-C） in serum and MDA,Hyp,SOD,GSH-Px,MMP-1 and TIMP-1 activity in hepatic tissue were detected. RESULTS： Compared with the model group,liver function and hepatic fibrosis were improved in drug groups. CONCLUSION： Apigeninnanoemulsion has a significant effect on the liver fibrosis rats,its mechanism is probably related to resisting-lipid peroxidation and adjusting the expression of MMP-1 and TIMP-1.
作者
庄让笑
潘旭旺
方红英
王福根
邵益丹
史婷婷
席建军
潘红英
ZHUANG Rangxiao PAN Xuwang FANG Hongying WANG Fugen SHAO Yidan SHI Tingting XI Jianjun PAN Hongying(Hangzhou Xixi Hospital Affiliated to Zhejiang Chinese Medical University, Hangzhou 310023, Zhejiang, China Zhejiang Provincial People＇s Hospital, Hangzhou 310014, Zhejiang, China)
出处
《中国临床药理学与治疗学》
CAS
CSCD
2016年第10期1122-1125,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
浙江省中医药科技计划项目(2012ZA109)
杭州市卫生科技计划项目(2012Z011)
