摘要
目的研究杨梅酮(myricetin,MTN)对高脂膳食小鼠学习记忆能力的影响。方法 64只C57BL/6J雄性小鼠随机分为对照组(CN,基础饲料),杨梅酮对照组(MTN,基础饲料+0.08%杨梅酮),高脂组(HF,高脂饲料),高脂+0.08%杨梅酮组(HF+MTN)4组。6w后采用Morris水迷宫测定小鼠空间学习记忆能力。以基因芯片分别探究高脂膳食与杨梅酮对海马信号通路的影响,q PCR法验证基因芯片结果。检测小鼠海马的氧化应激指标、炎性反应指标和BDNF的水平和相对表达量。结果与CN组相比,HF组小鼠学习记忆能力和BDNF水平显著下降(P<0.05);海马SOD和GSH-Px活性显著下降,MDA水平显著升高(P<0.05),但TNF-α、IL-6和MCP-1水平无显著变化(P>0.05)。长时程增强信号通路和过氧化物酶体信号通路是高脂膳食和杨梅酮共同改变的信号通路(P<0.05),高脂诱导海马Sod2、Prdx1、Prdx5、Creb、Itpr1和Plcb3表达显著下降。HF+MTN组认知障碍显著改善,海马SOD活性显著升高,MDA水平显著降低,显著上调Prdx1、Prdx5、Creb和Itpr1的表达(P<0.05)。结论杨梅酮通过维持海马氧化还原状态,调节长时程增强信号通路,可改善小鼠的学习记忆能力。
Objective To study the ameliorative effects ofmyricetin （MTN） on hippocampus-dependent learning and memory deficits in mice fed a high-fat diet. Methods Sixty-four male C57BL/6J mice were randomly divided into four groups： control group （CN）, CN＋0.08% MTN group （MTN）, a high-fat diet group （HF）, HF＋0.08% MTN group （HF＋MTN）. After 6 weeks, Morris water maze was used to evaluate spatial learning and memory. After sacrifice, microarray analysis was adopted to explore the effects of high-fat diet and myricetin on signaling pathways, which was validated by qPCR. The hippocampal oxidative stress related biomarkers, inflammatory biomarkers and brain-derived neurotrophic factors （BDNF） were determined. Results Compared to CN group, escape latency was increased. The time spent in the target quadrant and hippocampal BDNF levels were decreased in HF group （P〈0.05）, accompanied by significantly lower SOD and GSH-Px activities, elevated MDA levels and altered relative expressions ofPrdxl, PrdxS, Creb, ltprl and Plcb3 （P〈0.05）. However, no significance was observed for TNF-α, IL-6 and MCP-1 levels between CN and HF groups （P〉0.05）. Long term potentiation and peroxisome pathways were significantly altered by high-fat diet and myricetin （P〈0.05）. Myricetin could successfully improve hippocampus-dependent cognition, increase hippocampal SOD activity, decrease MDA levels and change the expressions of genes involved in learning, memory and oxidative stress （P〈0.05）. Conclusion Myricetin could maintain hippocampal redox status, regulate long term potentiation pathway, and improve learning and memory ability.
出处
《营养学报》
CAS
CSCD
北大核心
2016年第5期492-497,共6页
Acta Nutrimenta Sinica
基金
中央高校基本科研业务费专项资金资助(No.JUSRP115A33)
关键词
高脂
杨梅酮
氧化应激
学习记忆
high-fat diet
myricetin
oxidative stress
learning and memory
