摘要
【目的】以糖蜜发酵酒精废液为原料,探讨生化黄腐酸的高产发酵工艺技术参数,为生化黄腐酸高效生产提供理论依据。【方法】以白地霉、黑曲霉和短小芽孢杆菌的接种量、接种比例、培养基组成和发酵周期等因素进行单项和正交试验研究,采用硫酸铝絮凝质量法测定发酵液中生化黄腐酸的含量,探索发酵液中生化黄腐酸的含量最高的发酵条件及培养基。【结果】在总接种量为5%的条件下,白地霉、黑曲霉和短小芽孢杆菌的不同接种比例的生化黄腐酸含量为221.2~236.5 g·L^(-1),其中以1∶1∶2最佳;添加葡萄糖量在低于50.0 g·L^(-1)时,随着葡萄糖添加量的增加其生化黄腐酸含量也显著增加,但大于50.0 g·L^(-1)后,生化黄腐酸含量增加不显著;添加尿素含量在低于15.0 g·L^(-1)时,随着尿素添加量的增加其生化黄腐酸含量也显著增加。【结论】白地霉∶黑曲霉∶短小芽孢杆菌为1∶1∶2的比例下,接种量为6%的条件下,葡萄糖、尿素和硫酸铵含量分别为50.0,15.0,2.0 g·L^(-1);发酵周期为72 h的条件下,生化黄腐酸产量达到最高水平,为278.9 g·L^(-1)。几个因素对生化黄腐酸产量的影响程度大小顺序依次为:葡萄糖加量>发酵周期>尿素加量>接种量>硫酸铵加量。
【Objective】This research was aimed to study the technical parameters for efficient production of biochemical fulvic acid（ BFA） by fermentation of Molasses Alcohol Wastewater and provide a theoretical basis for the efficient production of BFA. 【Method】Single-factor and orthogonal experiment were applied to test effects of inoculation amount and inoculation ratio of Geotrichhum candidum,Aspergillus niger and Bacillus pumilus,culture medium composition and fermentation period etc. on production of BFA. The aluminum sulfate flocculation gravimetric method was used for the determination of BFA. This study was conducted to identify fermentation conditions and culture medium of efficient production of BFA in fermentation broth. 【Result】In the inoculation amount of 5%,the BFA content produced by different proportion of Geotrichhum candidum,Aspergillus niger and Bacillus pumilus was 221. 2- 236. 5 g·L-（-1）,and the best proportion was 1∶ 1∶ 2. When the concentration of adding glucose was less than 15 g·L-（-1）,the of BFA content increased with the increase of glucose,but when it was more than 5%,the increase of BFA production was not significant. When the urea was less than 1. 5%,the content of BFA increased with the increase of urea. 【Conclusion】As the inoculation ratio of Geotrichhum candidum,Aspergillus niger and Bacillus pumilus was 1 ∶ 1 ∶ 2,inoculation amount was 6%,the content of glucose,urea and ammonium sulfate were 50. 0,15. 0 and 2. 0 g·L-（-1）,and the fermentation time was 72 h,the production of BFA was the highest,278. 9 g·L-（-1）. The effects of several factors on the production of BFA were decreased in the following order of： the concentration of glucose,the fermentation period,the amount of urea,the concentration of inoculation,the amount of ammonium sulfate.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2016年第10期89-95,共7页
Scientia Silvae Sinicae
基金
国家林业局引进国际先进林业科学技术项目(2013-4-54)
关键词
白地霉
黑曲霉
短小芽孢杆菌
混合发酵
Geotrichhum candidum
Aspergillus niger
Bacillus pumilus
coinstantaneous fermentation
