摘要
食源性致病菌是导致食源性疾病的重要爆发根源之一。分子生物学检测技术因其敏感性强、特异性高、快速简便、省时省力等优点,在食源性致病菌的鉴定与检测中扮演着重要角色。本文系统阐述了利用分子生物学技术检测食源性致病菌的方法,包括多重PCR、实时荧光定量PCR、环介导等温扩增技术、基因芯片、液相芯片和基因探针技术等,分析了目前国内外学者对于这些技术由单一检测向多重检测的突破,及实现快速、高通量检测食源性致病菌的应用研究成果,总结了各种检测技术的优缺点,旨在为未来更好地发展快速、高通量检测食源性致病菌的技术方法提供参考。
Foodbome diseases are mainly caused by foodbome pathogens.Molecular biological detection technology which possesses the advantages of high sensitivity,high specificity,rapid and easy,and time-saving,and so on plays an important role in identification and detection of food-bome pathogens.This paper elaborated systematically the molecular biology technique adopted in detection of food-borne pathogens,including traditional PCR,multiple PCR,real-time fluorescent quantitative PCR,loop-mediated isothermal amplification technology,gene chips,liquid phase chips,and gene probes,and analyzed the breakthrough of single to multiple detection and research results of rapid and high-throughput detection of food borne pathogens from studies at home and abroad.Finally,the paper summarized the advantages and disadvantages of various detection techniques for the purpose that providing a reference for the better development of rapid,high-throughput detection food-borne pathogens in the future.
出处
《食品安全质量检测学报》
CAS
2016年第9期3497-3502,共6页
Journal of Food Safety and Quality
关键词
分子生物学技术
食源性致病菌
检测方法
食品安全
molecular biological technology
foodborne pathogens
detection method
food safety
