摘要
目的建立UPLC法分析藤黄Garcinia hanburyi Hook.f.及其炮制品（高压制、豆腐制、清水制）中藤黄烯酸、表藤黄烯酸、藤黄酸、新藤黄酸含有量的变化。方法该植物乙腈提取液的分析采用UPLC HSS T3色谱柱（100 mm×2.1 mm,1.8μm）;流动相乙腈-0.1%甲酸（80∶20）;体积流量0.3 m L/min;检测波长360 nm。结果 4种成分在各自范围内均呈良好的线性关系（r〉0.999 3）,平均加样回收率100.9%～103.0%（RSD〈3.0%）。炮制后,藤黄酸和新藤黄酸的含有量均有所下降,分别在清水、豆腐制品中最低。同时,新生成了藤黄烯酸和表藤黄烯酸,两者含有量在清水制品中最高,高压制品中最低。结论清水制法有利于藤黄烯酸和表藤黄烯酸的生成。
AIM To establish a UPLC method for analyzing the variations of contents of gambogellic acid,epigambogellic acid,gambogic acid and neogambogic acid in Garcinia hanburyi Hook. f. and its processed products（ high pressure-processed,tofu-processed and water-processed）. METHODS The analysis of acetonitrile extract of this plant was performed on a UPLC HSS T3 column（ 100 mm × 2. 1 mm,1. 8 μm）,with the mobilephase comprising of acetonitrile-0. 1% formic acid at a flow rate of 0. 3 mL / min,and the detection wavelength was set at 360 nm. RESULTS The four constituents showed good linear relationships within their own ranges（ r 〉0. 999 3）,whose average recoveries were 100. 9%-103. 0%（ RSD 〈 3. 0%）. After processing,the contents of gambogic acid and neogambogic acid were decreased,which were the lowest in water-processed and tofu-processed products,respectively. Simultaneously,gambogellic acid and epigambogellic acid were newly generated,whose contents were the highest in water-processed product,and the lowest in high pressure-processed product. CONCLUSION Processing with water is beneficial to the generations of gambogellic acid and neogambogic acid.
出处
《中成药》
CAS
CSCD
北大核心
2016年第11期2435-2438,共4页
Chinese Traditional Patent Medicine
基金
江苏省中药炮制重点实验室开放课题(ZYPZ004)
关键词
藤黄
炮制
藤黄烯酸
表藤黄烯酸
藤黄酸
新藤黄酸
UPLC
Garcinia hanburyi Hook
f.processing
gambogellic acid
epigambogellic acid
gambogic acid
neogambogic acid
UPLC
