摘要
【目的】从一批广西普通野生稻材料中筛选出新的抗白背飞虱材料,为水稻抗性品种培育和栽培稻遗传改良提供优异的抗性资源。【方法】通过成株期鉴定法对218份普通野生稻进行白背飞虱抗性株的初步筛选,以Ptb33为抗性对照、9311为感性对照;利用苗期集团法对初步筛选到的抗性材料进行抗白背飞虱鉴定,并对部分抗性较好的材料进行重复鉴定,确定试验结果的可靠性。【结果】利用成株期鉴定法从218份普通野生稻材料中初步筛选出60份对白背飞虱具有一定抗性的材料,占总鉴定材料的27.5%。利用苗期集团法从60份普通野生稻材料中鉴定出抗性材料22份,其中高抗材料1份,占鉴定材料数的1.7%,中抗材料21份,占鉴定材料的35.0%;对22份抗性材料中平均抗性水平较高的9份材料的重复鉴定结果与前期鉴定结果一致。【结论】通过对218份广西普通野生稻的抗性筛选,获得22份对白背飞虱具有中抗以上水平的抗性资源,这些抗性材料可应用于水稻抗性品种培育和栽培稻遗传改良。
【Objective】New rice materials with resistance to white-backed planthopper(WBPH) were selected from Guangxi common wild rice materials,in order to provide basic materials for resistant varieties breeding and genetic improvement of cultivated rice.【Method】A total of 218 common wild rice materials were identified at adult-plant stage for preliminary screening with Ptb33 as resistant control and 9311 as susceptible control,so some WBPH-resistant lines were selected from them.And seedling bulk test was used to identify resistance of selected WBPH-resistant lines.Finally,some lines with high resistance to WBPH were re-identified by seedling bulk test,so as to verify reliability of test results.【Result】Sixty WBPH-resistant rice materials was selected from 218 common wild rice species through identification at adult stage,which accounted for 27.5%of total materials,and twenty-two of which was identified as WBPH-resistant materials in seedling bulk test.However,only one material was highly WBPH-resistant,accounting for 1.7%of identified materials,and twenty-one materials were moderately WBPH-resistant,accounting for 35.0%.Furthermore,the repetitive identification results showed that,among twenty-two materials,nine rice materials had high average resistance,this result was consistent with result of earlier identification.【Conclusion】Through resistance screening,twenty-two materials with moderate or high WBPH-resistance are selected from 218 common wild rice materials,which can be used for resistant varieties breeding and genetic improvement of cultivated rice.
出处
《南方农业学报》
CAS
CSCD
北大核心
2016年第10期1693-1697,共5页
Journal of Southern Agriculture
基金
国家自然科学基金项目(31360325)
广西自然科学基金项目(2013GXNSFGA019009)
广西科学研究与技术开发计划项目(桂科重14121001-1-5)
关键词
普通野生稻
白背飞虱
抗性鉴定
抗性资源
common wild rice
white-backed planthopper
resistance identification
resistant resource