摘要
目的:建立Illumina Miseq深度测序筛查酸奶中微生物的方法,分析酸奶中微生物的多样性。方法:以9种酸奶样品为研究对象,提取酸奶中细菌基因组DNA,应用高通量测序技术测定细菌的16S r DNA v1-v3变异区序列,得到9种样品中微生物群体分布和丰度、以及不同样品间的菌种差异与进化关系。结果:酸奶中微生物主要为厚壁菌门(Firmicutes),占据99.6%之高,其中厚壁菌门主要以链球菌属(Streptococcus、87.1%)、乳杆菌属(Lactobacillus、10.3%)、乳球菌属(Lactococcus、0.3%)组成,9种酸奶中有3种同时含有链球菌属和乳杆菌属,其余6种样品中链球菌属几乎占据全部(>97%)。结论:Illumina Miseq深度测序技术可快速精确深入掌握酸奶中微生物多样性,结果对比显示不同酸奶菌种同质化程度高,其中链球菌属占绝对优势,同时发现部分酸奶标签标示不符。
Objective： Establishing a method based on Ⅲumina Miseq deep sequencing to analyze the microbial diversity of yogurt. Methods- Took 9 kinds of yogurt samples as the research objects and extracted the whole genome of bacteria of yogurt. The vl- v3 variation region of ldS rDNA were detected by high throughput sequencing technology to obtain the distribution and abundance of microbial community in 9 samples as well as the species difference and evolution relationship of bacteria among these samples.Results：The results showed that the main microbes of the yogurt were Firmicutes （99.6%） which mainly composed by Streptococcus（ 8？.1% ）, Lactobacillus（ 10.3% ）, and Lactococcus （0.3%） .There were 3 kinds of yogurt containing Streptococcus and Lactobacillus with a high number, Streptococcus （97%）accounted for almost all in the other six samples. Conclusion.Using Ⅲumina Miseq deep sequencing technology can master the microbial diversity of yogurt quickly and accurately. The results showed that the bacteria of yogurt were highly similar and streptococcus took a dominant position.At the same time, it was found that some yogurt labels didn＇ t match the analysis results well.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第24期78-82,共5页
Science and Technology of Food Industry
基金
长三角地区功能性微生态制剂关键检测技术及溯源体系的开发应用(1503062007)
长三角地区微生态制剂关键检测技术及溯源体系的开发应用(14495810200)
