摘要
羟基磷灰石因有良好的生物学活性被广泛应用于人工骨替代材料,但纯羟基磷灰石存在的自身不足限制了其临床应用范围。人体硬组织中含有多种具有重要生化作用的微量元素,本研究按照骨骼中微量元素含量对羟基磷灰石进行掺杂改性,制备出一种新型仿骨成分多元掺杂纳米结构羟基磷灰石材料(HA_2)。通过碱性磷酸酶活性染色、半定量PCR测量和茜素红染色等不同方法对比了未掺杂纳米结构羟基磷灰石(HA_1)和多元掺杂纳米结构羟基磷灰石材料(HA_2)对小鼠成骨前体细胞(MC3T3-E1)增殖及分化的影响。碱性磷酸酶活性染色实验证明HA_2比HA_1具有更强的促进细胞产生ALP的作用;半定量PCR测量证明HA_2比HA_1对成骨基因的表达有更为显著的促进作用;茜素红对矿化结节的染色实验同样证明HA_2比HA_1更有利于成骨细胞发生矿化。相对于未掺杂纳米结构HA_1,多元掺杂纳米结构HA_2对小鼠成骨前体细胞MC3T3-E1的分化及成骨有显著促进作用。多元掺杂改性使人工合成纳米结构羟基磷灰石更接近人体骨的无机成分,具有优良的成骨性能,是一种有前途的硬组织植入体材料。
Hydroxyapatite （HA） has been widely used as artificial bone substitutes because of its good biological activity, but the disadvantages of pure HA have limited its clinical applications. In human natural hard tissues, there are many trace elements doped in hydroxyapatite, which play beneficial roles in biological actions. In this study, multi-doped nanostructured hydroxyapatite （HA2） was synthesized by doping trace elements in HA according to the contents in human bones. Alkaline phosphatase activity staining, semi-quantitative PCR measurement （ALP, OCN and OPN） and alizarin red staining were carried out to detect the effect of pure nanostructured hydroxyapatite （HA1） and multi-doped nanostructured hydroxyapatite （HA2） on proliferation and differentiation of MC3T3-E lcells. Alkaline phosphatase activity experiments prove that the HA2 has a stronger role than HA1 in promoting cell production. Semi quantitative PCR measurement confirms that HA2 is more effective than HA1 in promoting the expression of osteogenic genes. Alizarin red staining results show that HA2 is better than HA1 for MC3T3-E1 ceils to produce mineralized nodules. Multiple-doped nanostructured hydroxyapatite （HA2） has an obvious promoting effect on the differentiation and osteogenesis of MC3T3-E1 comparing with the undoped nanostructured HA1. Multi-doped na- nostructured hydroxyapatite （HA2） is more beneficial to bone formation, which has the inorganic composition more close to human bone and is a promising biomaterial for human hard tissue implants.
出处
《无机材料学报》
SCIE
EI
CAS
CSCD
北大核心
2016年第12期1341-1346,共6页
Journal of Inorganic Materials
基金
国家自然科学基金(51232007)~~
关键词
羟基磷灰石
微量元素
多元掺杂
纳米结构
成骨活性
hydroxyapatite
trace elements
multi-doping
nanostructure
osteogenic properties
