摘要
目的:探讨熊果酸(ursolic acid,UA)是否通过调节miRNA-133a影响肺癌A549细胞的迁移和侵袭能力。方法:MTT法检测细胞活力;real-time PCR法检测UA干预和转染miRNA-133a mimics及inhibitor后细胞中miRNA-133a的表达;划痕愈合实验检测细胞的迁移能力;Transwell小室实验检测细胞侵袭能力。结果:UA可显著抑制肺癌A549细胞的活力(P<0.05),随着给药剂量的增加,UA对肺癌细胞的生长抑制率显著上升,并呈一定的剂量依赖关系,UA作用于肺癌A549细胞24 h后的IC50为31.04μmol/L。在不同浓度的UA作用下,肺癌A549细胞的迁移和侵袭能力也受到明显抑制(P<0.01)。Real-time PCR实验结果显示,在10、20μmol/L UA作用24 h后,A549细胞内miRNA-133a的表达显著高于溶剂对照组(P<0.01);细胞瞬时转染miRNA-133a mimics后可上调A549细胞中miRNA-133a的表达,而转染miRNA-133a inhibitor后下调A549细胞中miRNA-133a的表达(P<0.01)。肺癌A549细胞转染miRNA-133a mimics后,细胞活力、迁移和侵袭能力也受到明显抑制(P<0.01);相反,转染miRNA-133a inhibitor后,细胞活力、迁移和侵袭能力明显增强(P<0.01)。结论:UA能抑制肺癌A549细胞活力、迁移和侵袭,其作用机制可能是通过上调miRNA-133a介导来实现的。
AIM：To investigate the effects of ursolic acid（ UA） on the migration and invasion of human lung cancer cell line A549,and to explore its mechanism.METHODS：The cell viability was detected by MTT assay.The expression of miRNA-133 a was detected in the A549 cells treated with UA by real-time PCR.The miRNA-133 a mimics and inhibitor were transfected into the A549 cells,and the transfection efficiency was analyzed by real-time PCR.The cell migratory and invasive abilities were determined by wound healing and Transwell methods,respectively.RESULTS：The viability of the human lung cancer A549 cells was significantly inhibited by UA in a dose-dependent manner（ P〈0.05）.IC50 of UA（ 24 h） for lung cancer A549 cells was 31.04 μmol/L.UA treatment significantly inhibited the migratory and invasive abilities of A549 cells in a concentration-dependent manner,accompanied by significantly elevation of miRNA-133 a expression.The mimics and inhibitor of miRNA-133 a significantly upregulated and downregulated the expression of miRNA-133 a in the transfected A549 cells,respectively.In addition,the viability of the A549 cells was decreased extremely after tansfected with the miRNA-133 a mimics（ P〈0.01）,so did the results of the cell migration and invasion test.The A549 cells tansfected with the miRNA-133 a inhibitor showed an opposite changes of the cell viability,migration and invasion.CONCLUSION：UA inhibited the viability,migration and invasion of lung cancer A549 cells by elevating the expression of miRNA-133 a.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2016年第12期2239-2244,共6页
Chinese Journal of Pathophysiology
