摘要
目的观察颅针对脑缺血再灌注损伤大鼠脑梗死面积、血浆中SOD活性、MDA及NO含量的影响。方法将SPF级SD雄性大鼠48只,随机分成假手术组、模型组及颅针组。采用线栓法制备大鼠脑中动脉缺血(MCAO)模型,采用Longa EZ神经功能评分方法评价大鼠神经功能,采用TTC染色的方法测定大鼠脑梗死面积,并采用比色法检测血浆中超氧化物歧化酶(SOD)活性、丙二醛(MDA)及一氧化氮(NO)的含量。结果与假手术组相比,在第1、3、7天模型组大鼠的神经行为学评分均显著降低(P<0.01)。经TTC检测发现颅针组脑梗死体积显著小于模型组。同时与假手术组相比,模型组大鼠血浆SOD活性降低(P<0.01),MDA和NO含量升高(P<0.01);与模型组比较,颅针治疗后,血浆SOD活性升高(P<0.01),MDA、NO含量降低(P<0.01)。结论颅针对大鼠脑缺血再灌注损伤具有保护作用,能提高脑缺血再灌注损伤大鼠血浆SOD活性,降低血浆MDA、NO含量。
Objective To investigate the effect of skull acupuncture on cerebral infarct volume and plasma SOD activity and MDA and NO contents. Method Forty-eight male SPF-grade SD rats were randomized into sham operation, model and skull acupuncture groups. A rat model of middle cerebral artery occlusion （MCAO） was made by intraluminal thread occlusion. Rat nerve function was assessed by Zea Longa neurological function scoring. Rat cerebral infarct volume was measured by TTC staining. Plasma superoxide dismutase （SOD） activity and malondialdehyde （MDA） and nitric oxide （NO） contents were measured by colorimetry. Result Rat neurobehavioral score was significantly lower on day 1, 3 and 7 in the model group than in the sham operation group （P〈0.01）. TTC measurement showed that cerebral infarct volume was significantly smaller in the skull acupuncture group than in the model group （P〈0.01）. Plasma SOD activity was lower and MDA and NO contents were higher in the model group than in the sham operation group （all P〈0.01）. Plasma SOD activity increased and MDA and NO contents decreased after skull acupuncture treatment compared with the model group （all P〈0.01）. Conclusion Skull acupuncture has a protective effect against cerebral ischemia/reperfusion injury and can increase plasma SOD activity and decrease plasma MDA and NO contents in rats with cerebral ischemia/reperfusion injury.
出处
《上海针灸杂志》
2016年第12期1473-1476,共4页
Shanghai Journal of Acupuncture and Moxibustion
基金
福建省教育厅B类课题(JB09127)
关键词
电针
卒中
缺血再灌注损伤
氧化应激
大鼠
头针
颅针
Electroacupuncture
Stroke
Cerebral ischemia/reperfusion injury
Oxidative stress
Rats
Head acupuncture
Skull acupuncture
