摘要
为发掘果蔗抗病基因,促进果蔗抗病分子机制的研究,并为后续通过基因聚合分子育种提高果蔗品种的广谱抗性奠定基础,本研究根据已克隆的植物抗病基因保守结构域设计简并引物,采用RT-PCR方法对抗病果蔗品种黔糖5号的RNA进行扩增,共获得7条果蔗富亮氨酸重复的核苷酸结合位点(nucleotide binding site-leucine rich repeat,NBS-LRR)类抗病基因同源序列。氨基酸序列比对分析结果表明,这7条果蔗NBS-LRR类抗病基因同源序列均具有NBS典型结构域,且彼此间在氨基酸水平上表现出丰富的多态性。与7个已克隆的典型植物抗病基因同源序列构建系统进化树,7条果蔗抗病基因同源序列与基因RPM1和RPS2的亲缘关系较近,需进一步进行基因全长克隆和功能验证来确定含有这些片段的基因功能。
In order to mine resistance genes from chewing cane, promote molecular mechanism research of disease resistance in chewing cane and lay the foundation for improving the broad-spectrum resistance of sugarcane varieties by gene pyramiding, degenerate primers were designed based on the conserved domain of cloned resistance genes and RNA from Qiantang 5 were amplified using RT-PCR method and 7 NBS-LRR resistance gene analog(RGA) from fruit sugarcane were obtained. Amino acid alignment analysis showed that the7 nucleotide binding site-leucine rich repeat(NBS-LRR) RGAs possess classic NBS domain and rich diversity in amino acid sequences. The phylogenetic analysis of 7 NBS-LRR RGAs from the chewing cane and 7 cloned resistance genes were performed, and the result showed 7 chewing cane NBS-LRR RGAs had close kinship to genes RPM1 and RPS2. Full-length gene cloning and gene function studying will be needed to determine the functions of genes containing these RGAs.
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第2期352-358,共7页
Molecular Plant Breeding
基金
贵州省科学技术基金项目(黔科合J[2012]2253号
黔科合J[2013]03号)
贵州省农业公关项目(黔科合NZ[2012]3015号)共同资助
关键词
果蔗
抗病基因
富亮氨酸重复的核苷酸结合位点
抗病基因同源序列
Chewing cane
Resistance gene
Nucleotide binding site-leucine rich repeat(NBS-LRR)
Resistance gene analog
