摘要
目的探讨中药白花蛇舌草对Renca肾癌细胞模型小鼠凋亡相关蛋白Fas、caspase3及caspase7表达的影响。方法选取BALB/C小鼠120只,随机分为正常对照组、模型对照组、白细胞介素组、白花蛇舌草组,各30只,雌雄各半。除正常对照组外,各组均建立肾癌模型,并给予相应的药物治疗。观察所有小鼠的肿瘤体积及重量、抑瘤率,对Fas、Fas L、caspase3及caspase7蛋白表达水平进行检测。结果与模型对照组比较,白花蛇舌草组、白细胞介素组小鼠的肿瘤体积、瘤体重量较低,抑瘤率较高,Fas阳性表达率及Fas蛋白表达较高,Fas L阳性表达率及Fas L蛋白表达较低,caspase3、caspase7阳性表达率及蛋白表达水平较高,差异均有统计学意义(P<0.05)。其中白花蛇舌草组以上指标均优于白细胞介素组(P<0.05)。结论白花蛇舌草能够明显促进肾癌模型小鼠凋亡相关蛋白Fas、caspase3及caspase7表达,抑制Fas L蛋白表达,提高抑瘤率。
Objective To analyse the effect of hedyotisdiffusa on expressions of apoptosis related protein Fas,caspase3 and caspase7 in Renca renal cell carcinoma of model mice. Methods One hundred and twenty BALB / C male mice were randomly divided into normal control group,model control group,interleukin group,hedyotisdiffusa group,30 mice in each group,half male and female. Excepted for normal control group,renal cell carcinoma models were established in the other groups,and were given corresponding drug treatment. The tumor size and weight,and the tumor inhibition rate of all mice were observed,and the expression levels of Fas,Fas L,caspase3 and caspase7 were detected. Results Compared with model control group,the tumor size and weight were lower,the tumor inhibition rate were higher,the positive expression rates and protein levels of Fas were higher,the positive expression rates and protein levels of Fas L were lower,the positive expression rates and expression levels of Caspase3,Caspase7 protein were higher in the mice of interleukin group and hedyotisdiffusa group,all with significant differences（ P〈0. 05）. The above indicators in hedyotisdiffusa group were significant improved compared with interleukin group（ P〈0. 05）. Conclusion The hedyotisdiffusa can effectively promote the expressions of apoptosis related protein Fas,caspase3 and caspase7 in renal cell carcinoma of model mice,inhibit the expression of Fas L protein,improve the tumor suppression rate.
作者
周进
赵朋
ZHOU Jin ZHAO Peng(Department of Urological Surgery,Tianjin Nankai Hospital, Tianjin 300100, China)
出处
《中国生化药物杂志》
CAS
2016年第12期37-40,45,共5页
Chinese Journal of Biochemical Pharmaceutics
