摘要
目的通过检测干眼症(DES)患者结膜上皮细胞和泪液中肿瘤坏死因子-α(TNF-α)和胸腺基质淋巴细胞生成素(TSLP)的表达,初步探讨TNF-α和TSLP在DES发病的机制中的作用。方法分别选取2012年5月至2014年12月在我院眼科就诊的干眼症患者45例,经眼科体检未见异常且无全身免疫性疾病的健康志愿者45例,分成DES组及健康对照组,采集两组结膜上皮细胞并利用酶联免疫吸附试验(ELISA)法检测TNF-α和TSLP的表达水平;采集两组泪液并利用ELISA法检测泪液中TNF-α、TSLP表达水平。结果与健康对照组相比,DES组结膜上皮细胞中TNF-α、TSLP表达明显强于健康对照组,差异具有统计学意义(P<0.05);与健康对照组相比,DES组泪液中TNF-α和TSLP表达明显高于健康对照组,差异具有统计学意义(P<0.05)。结论 TNF-α和TSLP有可能参与了干眼症的发病过程,具体机制有待进一步探讨。
Objective To test TNF-α and TSLP expression levels in conjunctival epithelial cells and tears for dry eye syndrome（ DES） patients and healthy volunteers; to explore the roles of TNF-α and TSLP in the pathogenesis of DES.Methods 45 patients with dry eye syndrome and 45 healthy volunteers were enrolled between May 2012 and December2014 in our hospital. Conjunctival epithelial cells and tear were collected and the expression of TNF-α and TSLP were tested by enzyme-linked immunosorbent assay（ ELISA）. Results Compared with healthy subjects,TNF-α and TSLP were significantly more highly expressed in conjunctival epithelial cells and in tears in DES patients（ both P〈0. 05）. Conclusions TNF-α and TSLP may be involved in the pathogenesis of dry eye.
出处
《临床眼科杂志》
2016年第6期496-498,共3页
Journal of Clinical Ophthalmology
