摘要
目的:对牡丹皮中Fe,Mn,Cu,Zn,Al,Pb,As,Cd等24种微量元素采用煎煮法进行提取。方法:使用0.45μm的微孔滤膜分离提取液中的悬浮态和可溶态,将可溶态通过大孔树脂(LSA-10),以去离水和甲醇洗脱,确定微量元素的无机态和有机态;模拟人体胃肠溶液,采用正辛醇-水系统分离微量元素的醇溶态和水溶态;通过ICP-MS技术(电感耦合等离子体质谱法),测定微量元素总量及各种形态。结果:方法的回收率为74.7%~118.2%,精密度RSD在1.3%~6.9%。24种微量元素的溶出率为2.0%~82.9%,可溶态在水中的比率为73.6%~99.5%,可溶态中80%以上微量元素以无机态和水溶态形式存在。结论:微量元素的形态分析及溶出特征为牡丹皮的临床应用提供理论依据。
Objective： To extract 24 trace elements from Moutan Corter such as Fe,Mn,Cu,Zn,Al,Pb,As and Cd by using decocting method. Method： Soluble state and suspension state in the extract were separated by 0. 45 μm microporous filtering film. Soluble state was eluted from water and methanol by macrororous adsorption resin（LSA-10） in order to determine the inorganic state and organic state of the trace elements. In simulated human gastrointestinal solution,n-octylalcohol-water allocation system was used to separate water-soluble state and alcohol-soluble state for the trace elements. The total contents of the trace elements and various forms were detceted by inductively coupled plasma mass spectrometry（ICP-MS）. Result： The recovery rate of this method was74. 7%-118. 2% for all the elements,and precision RSD was 1. 3%-6. 9%. The extractive rate of the elements was2. 0%-82. 9%,and the ratio of water-soluble state was 73. 6%-99. 5%. More than 80% of the trace elements in soluble state were in the form of inorganic state and water-soluble state. Conclusion： The morphological analysis and dissolution characteristics provide theoretical basis for clinical application of Moutan Corter.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第3期39-44,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"重大新药创制"科技重大专项(2013ZX09402203)
关键词
牡丹皮
形态分析
溶出特征
ICP-MS
Moutan Corter
morphological analysis
dissolution characteristics
