摘要
目的:探讨和厚朴酚对鼻咽癌细胞株CNE-1的增殖、迁移与侵袭能力的影响,并研究其初步机制。方法:采用MTT法检测不同浓度的和厚朴酚(0、20、40、80与160μmol/L)对CNE-1细胞存活率的影响;采用划痕实验和Transwell小室检测细胞的迁移和侵袭能力;采用Western Blot检测PTEN、p-Akt与p-mTOR蛋白表达水平。结果:MTT法检测结果显示,和厚朴酚呈浓度依赖性抑制Eca-109细胞的生长,当浓度到达80μmol/L时,抑制作用达到最大;和厚朴酚干预后,CNE-1细胞迁移和侵袭能力显著下降,PTEN蛋白表达水平显著上调,p-Akt与p-mTOR蛋白表达水平显著下降。结论:和厚朴酚具有抑制鼻咽癌细胞株CNE-1增殖、迁移和侵袭的作用,其作用机制可能与和厚朴酚抑制PTEN/PI3k/Akt信号通路有关。
Objective：To investigate the effects of Honokiol on proliferation,migration and invasion in nasopharyngeal carcinoma cell line CNE-1and to explore the possible mechanism.Method：Cell viability was assessed using MTT in CNE-1cells treated with different concentrations of Honokio（0,20,40,80 and 160μmol/L）.Cell migration and invasion were analyzed by wound healing and Transwell assay.The protein expression of PTEN,p-Akt and p-mTOR were determined by Western Blot.Result：MTT assay showed that Honokiol decreased CNE-1cell viability in a concentration-dependent manner.The maximal inhibitory effects reached at 80μmol/L.The ability of CNE-1cell migration and invasion were significantly inhibited after Honokiol treatment.Moreover,western blotting revealed that Honokiol treatment resulted in an increase of PTEN protein expression and a decrease of pAkt and p-mTOR expression.Conclusion：Honokiol inhibits nasopharyngeal carcinoma cell line CNE-1proliferation,migration and invasion via inactivation of PTEN/PI3k/Akt signaling pathway.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
北大核心
2017年第2期88-90,95,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
