摘要
目的研究柚皮苷的体外抗肿瘤活性。方法采用MTT法测定柚皮苷对5种不同肿瘤细胞的增殖抑制作用。采用流式细胞术检测柚皮苷对细胞凋亡、细胞活性氧水平及细胞线粒体膜电位水平的变化。采用单细胞凝胶电泳(SCGE)实验检测柚皮苷对细胞DNA的损伤。结果 MTT实验得出柚皮苷对不同的肿瘤细胞产生不同的增殖抑制现象,并且对肿瘤细胞的抑制作用呈浓度依赖性。其对PC-12细胞作用24 h后,流式细胞术检测得到柚皮苷能有效诱导PC-12细胞的凋亡,并且细胞内活性氧水平升高,线粒体膜电位下降。SCGE实验发现柚皮苷使细胞DNA断裂,形成拖尾。结论柚皮苷通过活性氧调控的线粒体途径诱导肿瘤细胞PC-12的凋亡。
Objective To study the anticancer activity of naringin in vitro. Methods MTT assay was used to detect the growth inhibition of naringin on human cancer cell lines. Flow cytometry was used to analyze the cell apoptosis,reactive oxygen species and the changes of mitochondrial membrane potential. DNA damage was investigated by single cell gel electrophoresis（ SCGE）. Results Treatment with naringin inhibited the proliferation of PC-12, SGC-7901, Bel-7402, Hep G-2 and Si Ha cells in a dose-dependent way. Naringin induced cell apoptosis,increased ROS levels and decreased the mitochondrial membrane potential in PC-12 cells. Additionally,naringin induced DNA damage in PC-12 cells. Conclusion Naringin may induce PC-12 cell apoptosis through modulation of ROS-mediated mitochondrial pathway.
出处
《广东药学院学报》
CAS
2016年第6期757-761,共5页
Academic Journal of Guangdong College of Pharmacy
