摘要
[ Objective] To develop biological control resources of endophytic bacteria in wheat, the bacteria with antagonistic effect against Fusarium graminearum were screened and their antagonistic activity was studied. [ Method] The endophytic bacteria of wheat with antagonistic effect were screened using plate confrontation method; the antagonistic bacteria were identified through physiological and biochemical characteristics and 16S rDNA sequence analysis; the antagonistic spectrum of antagonistic bacteria was studied using plate confrontation method. [ Result] The antagonistic bacterium XY-3 was isolated from wheat leaves, and was identified as Bacillus amyloliquefaciens according to the physiological and biochemical characteristics and 16S rDNA sequence analysis. [ Conclusion] XY-3 had antagonistic effect against four kinds of common pathogenic fungi, presenting broad spectrum antagonistic activity against plant pathogenic fungi.
[ Objective] To develop biological control resources of endophytic bacteria in wheat, the bacteria with antagonistic effect against Fusarium graminearum were screened and their antagonistic activity was studied. [ Method] The endophytic bacteria of wheat with antagonistic effect were screened using plate confrontation method; the antagonistic bacteria were identified through physiological and biochemical characteristics and 16S rDNA sequence analysis; the antagonistic spectrum of antagonistic bacteria was studied using plate confrontation method. [ Result] The antagonistic bacterium XY-3 was isolated from wheat leaves, and was identified as Bacillus amyloliquefaciens according to the physiological and biochemical characteristics and 16S rDNA sequence analysis. [ Conclusion] XY-3 had antagonistic effect against four kinds of common pathogenic fungi, presenting broad spectrum antagonistic activity against plant pathogenic fungi.
基金
Supported by Special Fund for Agro-scientific Research in the Public Interest"Research and Demonstration of Key Prevention and Control Technology against Waterlogged Disasters in Major Crops"(201203032)
Innovative Experiment Project of College Students(20150086)
Open Fund of Hubei Collaborative Innovation Center for Grain Industry(2015MS020)
