摘要
目的:建立测定胃肠安胶囊中盐酸小檗碱含量的方法。方法:采用HPLC法,色谱柱:Symmetry C18(5μm,6×150 mm),流动相:乙腈-0.02 mol/L的磷酸二氢钾溶液(20∶80),流速:1.0 m L/min,检测波长:345 nm,进样量:10μL。结果:盐酸小檗碱在2.1675~86.70μg/m L范围内时,与峰面积呈良好的线性关系(R2=0.9996),平均回收率为87.88%。结论:HPLC法测定盐酸小檗碱含量方法简便,灵敏度高,重复性好,可用于胃肠安中盐酸小檗碱的含量测定。
Objective： To establish an HPLC method for determination of berberine hydrochloride content in Wei Changan Capsule （胃肠安胶囊, WCAC）. Methods： Symmetry C18（5 μm, 6mm×150mm）was the chromatographic column. The mobile phase was acetonitrile -0.02 mol/L monopotassium phosphate solution （20：80）. The flow rate was 1.0 mL/min, and the detection wavelength was 345 nm. The injection volume was 10 μL. Results： Within the range from 2.1675 to 86.70 μg/mL, berberine hydrochloride presented a good linear relationship with the peak areas （R^2=0.9996）. The average recovery was 87.88%. Conclusion： The method of HPLC to determine the content of berberine hydrochloride is simple, highly sensitive, and reproducible. It can be used for the quality of berberine hydrochloride content in wei changan capsule.
出处
《中医药导报》
2017年第2期64-66,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
"军队医疗机构制剂标准提高计划专项课题重点项目"-"成都战区云南区域非标准制剂质量提升关键技术的系统研究"(编号:14ZJZ18)
