摘要
【目的】研究沙冬青总黄酮体外抗牛副流感病毒Ⅲ型(BPIV-3)的作用,并对其抗病毒作用机制进行探讨.【方法】以细胞存活率和抑制率为指标,采用MTT比色法检测沙冬青总黄酮抗BPIV-3活性.分别以感染病毒同时给药、给药后接种病毒、先感染病毒后给药3种方式观察沙冬青总黄酮对BPIV-3病毒的抑制效果.【结果】沙冬青总黄酮的安全浓度为0.187mg/mL,病毒半数细胞感染量(TCID50)为10-7.733/0.1mL.在安全浓度范围内,沙冬青总黄酮具有良好的抗BPIV-3作用,且这种作用呈一定的量效关系.当浓度为0.187mg/mL时,BPIV-3抑制率达89.43%,细胞存活率高达96.66%;当浓度为0.187mg/mL时,阻断抑制率达68.36%,而细胞存活率达到86.98%;当浓度为0.187mg/mL时,对BPIV-3复制抑制效果最明显,达到50.55%,而细胞存活率达到78.81%.同时,在安全浓度范围内,沙冬青总黄酮直接杀灭BPIV-3病毒的效力较阻断作用和抑制作用更强.【结论】沙冬青总黄酮具有良好体外抗牛副流感病毒Ⅲ型(BPIV-3)的作用.
[Objective] To study the anti-viral effect of the total flavonoid of Amrnopiptanthus mongoli- ca against BPIV-3 virus in vitro and its antiviral mechanism. [Method] Cell survival rate and inhibition rate were used as index to evaluate antiviral activity of the total flavonoid of A. rnongolica by MTT. Cell surviv- al rate and inhibition rate were measured by three administration ways of infecting virus-and-applying hy- pericin,inoculating virus-after-applying hyperiein, infecting virus before applying hypericin, respectively. [Result] The results showed that safe concentration of total flavonoid of A. mongolica was 0. 187 mg/mL, BPIV-3 TCIDso was 10 7＂733/0~ 1 mL,within the concentration it had good inhibition effect on the replica- tion of BPIV-3 and showed certain dose-effect. Compared with the virus control group, at the concentration of 0. 187 mg/mL,BPIV-3 inhibition rate reached 89. 430//oo, cell viability up to 96.66 G. At the concentration of 0. 187 mg/mL, blocking inhibition rate reached 68. 36% and cell survival rate was 86. 98%. At the con- centration of 0. 187 mg/mL, replication inhibiting effect on BPIV-3 was most obvious reaching 50. 55 X and cell survival rate 78. 81G. The effect of direct killing on BPIV-3 was better than blocking and inhibiting effect. [Conclusion] The total flavonoid of A. mongolica has obvious effect against BPIV-3 virus in vitro.
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2016年第6期6-10,共5页
Journal of Gansu Agricultural University
基金
国家自然科学基金(31560686)项目
关键词
沙冬青总黄酮
抗病毒作用
BPIV-3
体外试验
total flavonoid of Ammopiptanthus mongolica
antiviral effect
BPIV-3 virus
in vitro test
