摘要
【目的】从新疆高放射土壤中分离和筛选出对苹果树腐烂病等有拮抗作用的放线菌,并对其进行鉴定。【方法】采用对峙培养法通过初筛、复筛测定拮抗菌对8种病原菌的拮抗效果,根据筛选出菌株CH10的形态特征、生理生化特性及16S rDNA序列对其进行鉴定。【结果】从70种放线菌中筛选出对苹果树腐烂病等8种新疆作物重要病原菌都具有良好拮抗效果的CH10。【结论】菌株CH10属于链霉菌属,对苹果树腐烂病菌等有良好的拮抗效果,为该病害的生物防治提供研究基础。
[ Objective] This project aims to isolate and screen 70 actinomycetes strains from the high ra- diation soil of Xinjiang by antagonistic effect of 8 kinds of pathogenic bacteria such as the Valsamali Miyabe et Yamada. [ Method ] By the plate confrontation culture method, and through initial screening and repeated screening of antagonistic effect of 8 kinds of pathogenic bacteria, and according to the morphological character- istics of the strains of CH10, the physiological and biochemical characteristics and 16S rDNA sequence went through identification and analysis. [ Result] The results showed that 8 kinds of important pathogens of Xinjiang crops, such as the Valsa malt Miyabe et Yamada, were screened out from 70 kinds of actinomycetes, which had good antagonistic effect called CH10. [ Conclusion] Strain CH10 belongs to the genus streptomyces and it has good antagonistic effect on the Valsa malt Miyabe et Yamada, which has provided a foundation for the bio- logical control of the disease.
出处
《新疆农业科学》
CAS
CSCD
北大核心
2017年第1期132-139,共8页
Xinjiang Agricultural Sciences
基金
新疆科技援疆项目(201491150)
高层次引进人才(2015-2017)
Supported by Key Projects in the National Science&Technology Pillar Program during the Twelfth Five-year Plan Period.(2012BAD42B02)~~
关键词
拮抗放线菌
病原菌
苹果腐烂病
16S
RDNA
链霉菌
antagonistic actinomycetes
pathogenic bacteria
16S rDNA identification
streptomyces
