摘要
目的:耐力训练引起的高脂膳食诱导肥胖大鼠Irisin水平变化与脂肪组织FTO表达的关系研究。方法:以雄性SD大鼠为研究对象,将大鼠随机分为对照组(C组)、高脂膳食组(HD)、高脂膳食结合运动组(HDE)和运动训练组(CE组),以跑台训练为运动干预手段,坡度为0°,8.2m/min(相当于53%VO2max),第1次运动15min,第2次运动30min,第3次运动45min,第4次以后每次运动60min,共运动8周,每周5次,测定大鼠体重、脂肪重量、血液Irisin含量、骨骼肌FNDC5mRNA和蛋白表达、脂肪组织FTO和UCP1基因mRNA和蛋白表达。结果:高脂膳食显著引起大鼠体重和脂肪含量增加,血液Irisin水平显著降低,骨骼肌FNDC5表达显著降低,脂肪组织FTO表达显著升高而UCP1表达显著降低;耐力训练可以显著降低高脂膳食引起的体重和脂肪含量的增加,显著升高高脂膳食引起的血液Irisin降低,显著升高高脂膳食诱导的骨骼肌FNDC5表达的降低,显著降低高脂膳食引起的脂肪组织FTO表达的升高,而显著升高高脂膳食诱导的UCP1表达的降低;血液中Irisin水平与脂肪组织中FTOmRNA和蛋白呈中度负相关;高脂膳食与高脂膳食结合运动组,骨骼肌中FNDC5mRNA和蛋白与脂肪组织FTOmRNA和蛋白分别呈中度负相关;运动组FNDCmRNA与脂肪组织FTOmRNA无相关,而蛋白呈中度负相关。结论:耐力训练大鼠可能通过释放Irisin,抑制脂肪组织FTO基因表达,进而增强UCP1表达。
Objective：To investigate the relationship between Irisinincduced by endurance training and FTO expression in fatty tissue in high fat diet - induced obese rats. Methods：Themale SDrats were randomly divided into control, high fat diet group, high fat diet plus exercise group and exercise group. Exercisewas performed on a treadmill, keeping 8.2 m/rain at 0% grade corresponding to 53% of maximal oxygen uptake ,for 15,30 , 45 and 60 min for the first, second and third time. Thereafter, exercise intensity and duration were maintained until the end of the eighth week. The rats were exercised for 5 days a week. It analysised the body weight, fat mass, blood Irisin level, FNDC5 expression in skeletal muscle and expressions of FTO and UCP1 in fatty tissue after 8 week treadmill training. Results：High fat diet significantly induced body weight and fat mass increase, blood Irisin level decrease, FNDC5 expression decline in skeletal muscle and FTO expression increase and UCP1 expression decrease in fatty tissue in rats. Endurance training can markedly decrease high fat diet -induced body weight and fat mass increase, increase hight fat diet - induced blood Irisin level decline, promote high fat diet - in- duced FNDC5 down - regulated expression in skeletal muscle and reduce high fat diet - induced FTO up - regu- lated expression but increase down - regulated UCP1 expression in fatty tissue. Blood Irisin level was negatively correlated to FTOmRAN and protein significantly in fat tissue in high fat diet, high fat diet plus exercise and exercise groups. FNDC5mRNA and protein in skeletal muscle were markedly negatively correlated to FTOmRNA and protein in fatty tissue respectively in high fat diet group and high fat diet plus exercise group. FNDC5mRNA in skeletal muscle was negatively correlated to FTOmRNA but no significance, however, FNDC5 protein was markedly negatively correlated with FTO protein in exercise group. Conclusion： Endrance training may be induce Irisin release increase, which inhibit FTO expression and then enhance UCPI expression in fatty tissuein rats.
出处
《成都体育学院学报》
CSSCI
北大核心
2017年第1期113-119,共7页
Journal of Chengdu Sport University
基金
山东理工大学青年支持计划项目"耐力训练改善肥胖的FTO机制研究"(110026)
关键词
耐力训练
肥胖
Ⅲ型肌肉纤维蛋白包含蛋白5
脂肪质量与肥胖相关基因
大鼠
Endurance Training
Obesity
FibronectinType III Domain Containingprotein 5
FatMass and Obesity - Associated Protein Gene
Rats
