摘要
中药质量评价分为有效性和安全性2个方面,现有的中药质量评价方法以仪器检测方法居多,不能满足生产及生活中简单、快速、现场化的实际需求。免疫检测技术具有灵敏度高、特异性强、操作简单、检测快速、检测成本低、仪器依赖性低、易于现场化等优点,在临床检验、食品安全检测及环境污染监测等领域应用广泛。近些年来,免疫检测技术逐渐应用于中药质量控制领域,涉及中药有效成分的定量检测,中药有害物质检测,中药外源性污染物检测等方面。该文综述了应用较广的酶联免疫检测法和胶体金免疫检测试纸条技术的原理及在中药质量评价中的应用,该技术在中药质量现场快速检测中具有较好应用前景。
The quality evaluation of traditional Chinese medicine can be divided into two aspects,effectiveness and safety. The existing methods for evaluating the quality of traditional Chinese medicine（ TCM） are mostly based on the testing instruments,which can not meet the practical needs of simple,rapid and on-site in production and life. Immunoassay,characterized by simple,rapid,sensitive,specific,low-cost and high-throughput,is widely used in the fields of clinical diagnosis,environmental pollution monitoring,food safety testing,and other fields. In recent years,immunoassay technology has been gradually applied in the field of quality control of TCM,involving quantitative detection of effective components of TCM,detection of harmful substances in TCM,and detection of exogenous pollutants in TCM. This paper summarizes the principle of the wide application of ELISA and colloidal gold immunostrip technology and its application in quality evaluation of TCM,this technique has a good application prospect in the field of rapid detection of the quality of TCM. In this paper,the principles of the widely used ELISA and colloidal gold immune test strip technology as well as their application in quality evaluation of TCM were reviewed,and the results showed that this technique had a good application prospect in the field of rapid detection of the quality of TCM.
作者
张波
南铁贵
孙晴
刘洪美
丁凤伟
王颖
ZHANG Bo NAN Tie-gui SUN Qing LIU Hong-mei DING Feng-wei WANG Ying(College of Pharmacy, Linyi University, Linyi 276000, China State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese MateriaMedica, China Academy of Chinese Medical Sciences, Beijing 100700, China Shandong C. P. Freda Pharm Co. Ltd. , Ji＇nan 250104, China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2017年第3期420-427,共8页
China Journal of Chinese Materia Medica
基金
丹参GAP方案的研制(HX150013)
