摘要
目的观察槐耳不同提取部位对人胃癌MKN-45细胞增殖的影响,并筛选其有效部位。方法用系统溶剂法将槐耳提取为石油醚、乙酸乙酯、正丁醇、乙醇、水五个部位后,用CCK-8显色法,检测不同部位不同浓度的槐耳醇提取物对MKN-45增殖的作用。结果槐耳石油醚、乙酸乙酯、正丁醇、乙醇部位为抗肿瘤有效部位,其中以正丁醇部位效果最佳,其对MKN-45细胞半数抑制率(IC50)为56.142μg·m L-1。结论槐耳醇提物能抑制人胃癌MKN-45细胞增殖,其最有效部位为槐耳正丁醇部位。
Objective To investigate the effects of different extracts from Huaier on the proliferation of human gastric cancer MKN-45 cells and to screen the effective parts. Methods Huaier were extracted by System solvent method to petroleum ether, ethylacetate, n-butanol, ethanol and water, CCK-8 method was applied to colour development. The effects of different concentrations of Huaier alcohol extract on MKN -45 proliferation were detected. Results The results showed that the chemical constituents of n-butanol were the best, and the IC50 of MKN-45 cells was 56. 142μg mL-1. Conclusion The ethanol extract of Sophora japonica can inhibit the proliferation of human gastric cancer MKN- 45 cells, and its most effective site is the locus of n-butanol.
作者
黄挺
陈佳辉
徐志远
吕航
程向东
Huang Ting Chen Jiahui Xu Zhiyuan Lv Hang Cheng Xiangdong(Zhejiang University of Chinese Medicine, Hangzhou 310053, China Zhejiang Provincial Hospital of Chinese Medicine, Hangzhou 310006, China)
出处
《陕西中医药大学学报》
2017年第1期73-75,92,共4页
Journal of Shaanxi University of Chinese Medicine
基金
国家自然科学基金(81573953)
关键词
槐耳
提取物
人胃癌MKN-45株
细胞增殖
Huaier
extract
human gastric cancer MKN-45 strain
cell proliferation
