摘要
【目的】分析Myh6基因在新西兰白兔Oryctolagus cuniculus肌肉中的表达规律及与肌纤维特性的相关性,探究Myh6基因在肌纤维发育过程中的作用。【方法】利用冰冻切片技术测量了2、4、6、8、10、12周龄新西兰白兔背腿肌中不同类型肌纤维的密度、面积、长径和短径等性状,采用qRT-PCR测量了Myh6基因在不同周龄新西兰白兔背腿肌中的表达量,分析了上述性状与Myh6基因表达的相关性。【结果】2~8周龄新西兰白兔各类型肌纤维的密度逐渐变小,面积、长径和短径则逐渐变大(白肌纤维短径除外,仅在2~6周龄有变大趋势)。Myh6基因在4周龄表达量最高。Myh6基因表达量与公兔背部红肌纤维的面积呈极显著负相关(P<0.01),与公兔腿部红肌纤维的密度呈极显著正相关(P<0.01)。【结论】Myh6基因表达与肌纤维类型和肌纤维的生长发育密切相关。
【Objective】To analyze Myh6 gene expression and its correlation with the muscle fiber traits of New Zealand white rabbits,and explore the role of Myh6 gene during the development of muscle fibers.【Method】Density,area,long and short diameters for back and leg muscle fibers were measured for 2-,4-,6-,8-,10-and 12-week-old New Zealand white rabbits using the frozen section technique. Myh6 gene expressions in back and leg muscles of rabbits at different ages were estimated using qRT-PCR. The correlations between Myh6 gene expression and muscle traits were analyzed. 【Result】The density of various muscle fibers gradually decreased,but the area,long and short diameters of muscle fibers increased from two to eight weeks,except for the short diameters of white muscle fibers increased from two to six weeks. The expression level of Myh6 gene was the highest in 4-week-old rabbit. The expression level of Myh6 gene was significantly（ P〈0. 01） negatively correlated with the red muscle fiber area of male rabbit back muscles,and significantly（ P〈0. 01） positively correlated with the red muscle fiber density of male rabbit leg muscles. 【Conclusion】Myh6 gene expression is closely related to type and growth of muscle fibers.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2017年第2期12-17,共6页
Journal of South China Agricultural University
基金
现代农业产业技术体系建设专项(CARS-44-1)
江苏高校优势学科建设工程资助项目(PAPD 2014-134)
关键词
兔
肌纤维
冰冻切片
Myh6
相关性分析
rabbit
muscle fiber
frozen section
Myh6
correlation analysis
