摘要
目的分析脱敏煎治疗男性免疫性不育患者前后AsAb、精液常规参数、外周血T细胞及Th细胞亚型分布的变化情况,探讨脱敏煎对男性免疫性不育患者的精液常规参数及机体免疫状态的影响。方法收集男性免疫性不育患者50例,在脱敏煎治疗前后检测患者AsAb;计算机辅助精子分析仪检测精子浓度及前向运动精子(PR);低盐膨胀实验检测精子存活率,Diff-Quick染色法检测正常精子形态百分率;流式细胞术检测患者外周血T细胞亚型分布;RT-PCR检测IFN-γ、IL-4 mRNA表达情况。结果经脱敏煎治疗后,患者AsAb转阴率达76.00%,精子浓度明显升高(P<0.05),精子活率及PR亦明显升高(P均<0.01),而正常精子形态百分率无明显变化(P>0.05)。患者外周血CD3^+T和CD8^+T细胞数明显降低,CD4^+T细胞数明显升高(P均<0.01)。IFN-γmRNA表达量显著下调(P<0.05),IL-4 mRNA的表达量显著上调(P<0.05)。结论脱敏煎治疗可明显改善男性免疫性不育患者T淋巴细胞功能失调,提高精子浓度/精子存活率和活力,降低AsAb阳性率。
Objective To investigate the changes of AsAb, semen routine parameters, peripheral blood T cells and Th cell subtypes in patients with male immunological infertility before and after treatment, and to explore the effects of desensitization decoction on semen routine and the immune status in patients with male immunized infertility. Methods A total of 50 patients with male immunological infertility were collected and AsAb Of them were detected before and after desensitization. The sperm concentration and the forward spermatozoa （PR） were measured by computer -assisted sperm analyzer. The survival rate of sperm was detected by low salt swelling experiment. Diff-Quick staining was used to de- tect the percentage of normal spermatozoa. Flow cytometry was used to detect the distribution of T cell subtypes in pe- ripheral blood of patients. And the expressions of IFN-γ and IL-4 mRNA were detected by RT-PCR. Results After treatment with desensitization decoction, the AsAb negative rate was 76.00% and the sperm concentration increased significantly（P〈O.05）. The sperm viability rate and PR also increased significantly（P〈0.01）, while the percentage of nor- mal spermatozoa had no significant change（P〉0.05）. The number of CD3＋T and CD8＋T cells in peripheral bloQd signifi- cantly decreased and the number of CD4CF cells in,creased significantly （P〈0.01）. IFN-γ mRNA expression was signifi- cantly down-regulated （P〈0.05）, and the expression of IL-4 mRNA was significantly up-regulated（P〈0.05）. Conclusion Desensitization decoction therapy can significantly improve the T lymphocyte dysfunction in male immunized infertility patients, and improve sperm concentration, sperm survival rate and viability, and can decrease the positive rate of AsAb.
出处
《中国现代医生》
2017年第3期15-17,21,共4页
China Modern Doctor
基金
浙江省宁波市自然基金项目(2016A610200)
关键词
男性免疫性不育
T细胞亚型
Th细胞极化
脱敏煎
Male immunological infertility
T cell subtype
Th cell polarization
Desensitization decoction
