摘要
目的探讨复方苦参与5-氟尿嘧啶(5-Fu)联合用药对结肠癌细胞增殖和凋亡的作用以及对细胞周期素1(CyclinD1)表达的影响。方法 CCK-8细胞增殖实验与流式细胞术对比联合用药组(复方苦参+5-Fu)与对照组(二甲基亚砜+5-Fu)处理后的结肠癌SW480细胞增殖与凋亡情况;蛋白免疫印迹法观察两组SW480细胞CyclinD1的表达情况。结果联合用药组处理后的SW480细胞的增殖能力弱于对照组,两组间48 h、72 h及96 h 3个时间点的细胞增殖抑制情况比较,差异有统计学意义(P<0.05)。联合用药组SW480细胞的凋亡率(12.7%)高于对照组(6.18%),Cyclin D1的表达弱于对照组,组间比较,差异均有统计学意义(P<0.05)。结论复方苦参联合5-Fu能抑制结肠癌细胞SW480的增殖,促进其凋亡,作用机制可能与其抑制CyclinD1的表达有关。
Objective To explore compound matrine combined with 5-fluorouracil（5-Fu） on the proliferation and apoptosis of colon cancer cell（ SW480） and the expression of CyclinD1. Methods To observe the proliferation and apoptosis of SW480 cell interfered in combined treatment group（ compound matrine combined 5-Fu） and the control group（ dimethyl sulfoxide combined 5-Fu） using CCK-8cell proliferation experiment and flow cytometry; the expression of CyclinD1 in SW480 cell interfered between the two groups using Western blot. Results The proliferation ability of SW480 interfered in the combined treatment group was lower than that in the control group,the proliferation status on 3 points of 48 hours,72 hours and 96 hours had statistically significant difference between the two groups（ P〈0. 05）. The apoptosis rate of SW480 cells in the combined treatment group（ 12. 7%） was higher than that in the control group（ 6. 18%）,the CyclinD1 expression of SW480 interfered in the combined treatment group was less than that in the control group（ P〈0. 05）. Conclusion Compound matrine combined with 5-Fu can significantly inhibit the proliferation of colon cancer cell（SW480） and accelerate the apoptosis of SW480 cell. The mechanism of curative effect on colon cancer may be related to inhibiting the expression of CyclinD1.
出处
《临床军医杂志》
CAS
2017年第2期156-158,共3页
Clinical Journal of Medical Officers
基金
辽宁省科学技术项目(2015020408)
