摘要
目的通过分析SPF级雄性自发性高血压(SH)大鼠和WKY大鼠的肺泡灌洗液成分、观察动物的一般指标,肺组织的病理学特征,研究正常血压对照组大鼠和自发性高血压模型组大鼠的肺部血管损伤及肺部实质细胞损伤的差异。方法将48只SPF级雄性SH大鼠和WKY大鼠随机分为4组,每组6只,分别为生理盐水对照组及低、中和高剂量组,采用气管灌注的方法进行染毒,收集肺灌洗液,分析肺泡灌洗液成分并观察肺组织的病理切片。结果染毒组动物的体质量与染毒前比较差异均有统计学意义(P<0.05),并且中、高剂量染毒组动物与染毒前比较均降低,差异有统计学意义(P<0.05);染毒组动物的呼吸与染毒前比较均增快,差异具有统计学意义(P<0.05);染毒组肺泡灌洗液白蛋白(ALB)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)及乳酸脱氢酶(LDH)的水平与对照组比较差异均有统计学意义(P<0.05),SHR高剂量组动物肺泡灌洗液中上述指标的水平高于相同剂量下的WKY,差异具有统计学意义(P<0.05)。通过病理学观察发现染毒后动物肺部组织有明显病理性改变。结论细颗粒物对大鼠肺部有损伤作用,而SHR比WKY对大气细颗粒物致肺损伤的作用更加易感。因此、高血压疾病可能是大气细颗粒物引起肺部损伤的危险因素之一。
Objective The components of alveolus lavage fluid,the general indexes and the pathological characteristics of the lung tissues in SPF male spontaneously hypertensive rats（SH）and WKY rats were analyzed in the normal control group and the spontaneously to explore the difference of pulmonary vascular injury and parenchymal cell injury between normal control group and spontaneously hypertensive rats.Methods Forty-eight SPF male SH rats and WKY rats were randomly divided into four groups：normal saline control group,low,moderate and high dose groups,and the lungs were collected by bronchial perfusion lavage fluid,analysis of alveolar lavage fluid composition and observation of lung tissue sections.Results There were significant differences in body weight between the two groups（P〈0.05）,and the animals in the middle and high dose groups were significantly lower than those before exposure（P〈0.05）.The animals respiration in the exposure group were faster than before exposure（P〈0.05）.The levels of albumin,alkaline phosphatase,acid phosphatase and lactate dehydrogenase in bronchoalveolar lavage fluid were significantly different between the exposure group and the control group.The levels of ALB,AKP,ACP and LDH in the alveolar lavage fluid of SHR high-dose group were higher than those of the same dose（P〈0.05）.Histopathological observation showed that the pathological changes of the lung tissue were obvious.Conclusion Fine particles on rats lung injury,and SHR than WKY on the role of fine particles of lung injury caused by air more susceptible.Therefore,hypertensive disease may be caused by atmospheric fine particles of lung injury risk factors.
作者
古力娜扎尔.吾甫勒
晓开提.依不拉音
Gulinazhaer Wufule Xiaokaiti Yibulayin(School of Public Health, Xinjiang Medical University, Urumqi 830011, China)
出处
《新疆医科大学学报》
CAS
2017年第5期617-620,共4页
Journal of Xinjiang Medical University
基金
新疆维吾尔自治区自然科学基金(20142011C004)
关键词
细颗粒物
自发性高血压大鼠
肺损伤
fine particulate matter
spontaneously hypertensive rats
lung injury
