摘要
20世纪90年代,中国引进了一批体色呈金黄色的草鱼(Ctenopharyngodon idellus),生产上俗称金草鱼。为了解金草鱼群体的遗传结构和遗传多样性,利用15个微卫星DNA标记对金草鱼群体与中国草鱼群体(长江水系的沅江群体、宁乡群体、洪湖群体和珠江水系的西江群体)进行遗传结构和系统进化分析。结果表明15个微卫星位点均具有较高的多态性,多态信息含量(PIC)为0.763~0.939。金草鱼群体的遗传多样性水平[期望杂合度(HE)=0.662]低于中国草鱼群体[HE=0.852~0.885]。遗传分化指数(FST)分析显示,金草鱼群体与沅江群体之间的遗传分化属于高度分化(0.15<FST<0.25),与其他3个草鱼群体之间属于中度分化(0.05<FST<0.15)。遗传距离分析显示,金草鱼群体与西江群体遗传距离(DA)最小(0.476 3),与沅江群体最大(DA=0.810 7)。基于遗传距离构建的NJ系统进化树显示,4个中国草鱼群体聚为一支,金草鱼群体单独为另一支。研究结果显示金草鱼群体的遗传多样性低于中国的草鱼群体,亲缘关系也较远。
old grass carp is a kind of grass carp （Ctenopharyngodon idellus） that was introduced from abroad in the 1990s. We used 15 microsatellite markers to analyze the genetic structure and genetic diversity of gold grass carp population and four grass carp populations [Yuanjiang （YJ）, Ningxiang （NX）, Honghu （HH） and Xijiang （XJ）] from the Yangtze River and the Pearl River of China. The results show that all the 15 microsatellite loci were highly polymorphic, with polymorphism information content （PIC） of 0.763~0.939. The expected heterozygosity （HE） of gold grass carp population was 0.662, lower than those of the four Chinese grass carp populations （HE=0.852~0.885）. The analysis of genetic differentiation index （FST） indicates that the FST between gold grass carp population and YJ, NX, HH, XJ populations were 0.157 2, 0.129 5, 0.147 5 and 0.114 4, respectively. Genetic distance analysis shows that the genetic distance between gold grass carp population and XJ population was the shortest （0.476 3） ,and was the longest with YJ population （0.810 7）. The NJ phylogenetic tree based on genetic distance shows that the four Chinese grass carp populations clustered together as one branch, and gold grass carp population as another. The result shows that the genetic diversity of gold grass carp population is lower than that of Chinese grass carp population, with relative distant genetic relationship.
出处
《南方水产科学》
CAS
CSCD
北大核心
2017年第2期51-58,共8页
South China Fisheries Science
基金
“十二五”农村领域国家科技计划项目(2012BAD26B02)
国家大宗淡水鱼产业技术体系建设“华南草鱼选育与分子辅助育种”(CARS-46-03)
广东省海洋渔业科技与产业发展专项(A201401A03)
广东省自然科学基金项目(2016A030313148)
关键词
草鱼
遗传结构
微卫星
遗传距离
杂合度
Ctenopharyngodon idellus
genetic structure
microsatellite
genetic distance
heterozygosity
