摘要
目的 观察Kai1基因在由结直肠息肉进展至结直肠癌,以及结肠癌侵袭转移过程中表达水平的变化,探讨其临床意义.方法 通过免疫组织化学法检测Kai1在40例正常结直肠黏膜组织、80例结直肠腺瘤、40例结直肠癌（伴淋巴结转移者25例;不伴淋巴结转移者15例）中的表达;通过实时荧光定量聚合酶链反应（FQ-PCR）方法探讨Kai1 mRNA的表达变化;并与临床病理特征比较分析.结果 免疫组织化学结果显示Kai1在结直肠癌的蛋白表达阳性18例（45.00%）,明显低于结直肠腺瘤组（73例,91.25%）及正常肠黏膜组（40例,100.00%）,差异有统计学意义（P=0.031）;FQ-PCR结果显示Kai1 mRNA在结直肠癌、结肠腺瘤及正常结直肠黏膜组织中表达依次增高,分别为2.038±0.933、9.983±4.337、12.260±2.182 （F=11.960,P=0.015）.Kai1蛋白和mRNA的表达与结直肠癌淋巴结转移相关（P=0.011）.结论 Kai1蛋白和mRNA表达下调与结直肠癌的发生、发展明显相关.
Objective To observe the expression and clinical significance of kang ai 1 （Kai1） in the process from the progression from colorectal polyps to cancer, invasion and metastasis.Methods Immunohistochemistry was used to examine the expression of Kai1 in the colorectal tissues, colorectal adenoma tissues and colorectal carcinoma tissues.Real-time fluorescent quantitative polymerase chain reaction （FQ-PCR） was used to detect Kai1 mRNA expression.Results The expression of Kai1 protein was positive in 18 cases of colorectal carcinoma （45.00%）, significantly lower than that in colorectal adenoma （91.25%） and colorectal tissues （100.00%）, respectively, with significant differences among them （P=0.031）.FQ-PCR showed that the expression of Kai1 mRNA in colorectal carcinoma, colorectal adenoma and normal colorectal mucosa was incresed by 2.038±0.933, 9.983±4.337 and 12.260±2.182 respectively （F=11.960, P=0.015）.The expression of Kai1 protein and mRNA had a significant correlation with the lymph node metastasis of patients with colorectal carcinoma （P=0.011）.ConclusionThe abnormal expression of Kai1 protein and mRNA is related with the carcinogenesis and the development of colorectal carcinoma.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2017年第3期485-487,共3页
Chinese Journal of Experimental Surgery
关键词
KAI1
结直肠癌
基因表达
Kang ai 1
Colorectal carcinoma
Gene expression
