摘要
建立了高效凝胶渗透色谱法测定配方奶粉中免疫球蛋白G(IgG)含量的方法。奶粉样品经pH 7的0.05 mol/LNa_2HPO_4溶液溶解,高速离心、过滤,滤液经protein G免疫亲和柱净化。采用Zorbax GF-450凝胶色谱柱(250 mm×9.4 mm,6μm)分离,流动相为pH 7的0.05 mol/L Na_2HPO_4溶液,流速1.0 m L/min,检测波长280 nm。免疫球蛋白G在0.2~5.0 mg/m L的含量范围内线性关系良好,相关系数为0.9991,方法检出限为0.1 mg/g,加标回收率为86.0%~117.7%,相对标准偏差(n=6)为3.8%~7.1%。方法适用于配方奶粉中免疫球蛋白G的定性、定量分析。
A high performance gel permeation chromatographic detection method was developed for the determination of Immunoglobulin G in milk powder. Samples were dissolved with pH 7, 0. 05 mol/L disodium hydrogen phosphate buffer; then fat was removed by high-speed centrifugation and filtration. The filtered liquor was purified by an immunoaffinity column. Chromatographic conditions were follows： using an Agilent ZORBAX GF -450 gel permeation chromatography column （250 mm × 9.4 mm, 6μm）with mobile phase of pH 7,0. 05 mol/L disodium hydrogen phosphate buffer at rate 1.0 mL/min, the column is at room temperature and the detection wavelength of 280 nm. Results showed that good linarites in the range of 0. 2 - 5.0 mg/mL （ r = 0. 9991 ） with the detection limit of 10 mg/100g. The recoveries ranged from 86. 0% to 117.7% with relative standard deviations of 3.8%-7. 1%. This method is simple, accurate and can be used for quantitative and qualitative analysis of Immunoglobulin G in milk powder.
出处
《分析试验室》
CAS
CSCD
北大核心
2017年第4期456-459,共4页
Chinese Journal of Analysis Laboratory
基金
国家质检总局科技计划项目(2016IK213)
陕西检验检疫局科技计划项目(陕K201605
201606)资助
关键词
凝胶色谱
配方奶粉
免疫亲和柱
免疫球蛋白G
Gel permeation chromatography
Formula milk powder
Immunoaffinity column
Quantitation of Immunoglobulin G
