摘要
以2个金铁锁四倍体材料(T1、T2)及1个二倍体金铁锁组培苗(CK)为试材,采用石蜡切片法,观察不同倍性金铁锁组培苗的茎、叶解剖结构差异。结果表明:不同倍性金铁锁组培苗叶及茎的相关性状均差异显著。不同倍性金铁锁均无明显栅栏组织和海绵组织的分化。四倍体金铁锁T1与T2叶片厚度分别是二倍体的1.77、1.60倍;叶主脉厚度分别是二倍体的2.34、1.67倍。四倍体金铁锁T2的茎直径横切大小为二倍体金铁锁的1.40倍,而T1和T2的茎导管横切大小分别是二倍体的1.81、1.23倍;四倍体T1和T2的茎中空横切大小分别是二倍体的1.80、1.73倍。可见,金铁锁经多倍化处理后,其茎、叶的解剖结构发生了显著变异。研究结果可为金铁锁多倍体育种提供参考依据。
Two tetraploid（T1,T2）Psammosilene tunicoides and one diploid Psammosilene tunicoides（CK）were used as materials to observe the anatomical structure difference in different ploidy with paraffin method.The results demonstrated that the significant difference were existed in stem and leaf anatomical structure of different ploidy P.tunicoides tissue culture seedlings.The different ploidy P.tunicoides tissue culture seedlings had no obvious differentiation on both palisade tissue and spongy tissue.The blade thickness of the polyploidy material of T1and T2were 1.77,1.60times of diploid;the thickness of leaf primary vein were 2.34,1.67times of the diploid;the diameter cross-cutting size of T2stem was 1.40times of the diploid,and the cross-cutting size of stem catheter of T1and T2were 1.81,1.23times of the diploid;the cross-cutting size of stem hollow in T1and T2were 1.80,1.73times of the diploid.Obviously,significant variation had taken place in the anatomical structure of stem and leaf after being polyploidized.Theoretical reference would be provided to Polyploid Breeding of P.tunicoides from these results.
出处
《北方园艺》
CAS
北大核心
2017年第8期160-164,共5页
Northern Horticulture
基金
西南林业大学科技创新基金资助项目(C16026)
西南林业大学林学一级学科建设资助项目
贵州省社发攻关资助项目(黔科合SY字[2015]3027号)
关键词
金铁锁
倍性
叶
茎
解剖结构
Psammosilene tunicoides
ploidy
leaf
stem
anatomical structure
