摘要
目的探讨中草药黄芩苷对人乳腺癌细胞株MCF-7的作用及此作用存在的内在机制。方法 0μmol/L(NC)、50μmol/L、100μmol/L、150μmol/L、200μmol/L、250μmol/L的黄芩苷作用于人乳腺癌细胞株MCF-7不同时间后,观察细胞形态,MTT法测黄芩苷对细胞增殖的影响,并用流式细胞仪检测黄芩苷是否诱导细胞的凋亡,最后用RT-PCR来检测凋亡相关基因m RNA的表达。结果在一定的时间和一定的黄芩苷药物浓度的范围内,黄芩苷能抑制人乳腺癌细胞株MCF-7的增殖和迁移并诱导人乳腺癌细胞株MCF-7的凋亡,50μmol/L组抑制MCF-7的迁移与对照组之间有统计学差异(P<0.05)。而且能引起ERK信号通路中凋亡相关基因m RNA表达量的变化。给药组的caspase-3和caspase-9被激活,50μmol/L组bcl-2、bax与对照组之间有统计学意义(P<0.05)。结论黄芩苷通过ERK这条信号通路激活caspase-3和caspase-9,下调bcl-2,上调p-ERK、bax、p38从而诱导乳腺癌MCF-7细胞凋亡。黄芩苷能够显现出较大的经济效益,且更重要的是能够为乳腺癌患者提供新的药物方案和治疗思路。
Objective To explore the internal mechanism of chinese herbal medicine baicalin in induction of apoptosis of human breast cancer cell line MCF-7. Methods After some time of 0 μ mol/L （NC）, 50 μ mol/L, 100 μ mol/L, 150 μ mol/L, 200μmol/L, 250 μL mol/L baicalin effected on human breast cancer cell line MCF-7, cell morphology was observed. MTT assay was used to detect the effect of Baicalin on cell proliferation. Flow cytometry was used to detect weather baicalin induced cell apoptosis or not. RT-PCR was used to detect the expression of apoptosis related gene mRNA. Results Within a certain period of time and a certain concentration of baicalin, baicalin could inhibit the proliferation and migration of human breast cancer cell line MCF-7 and induce the apoptosis of human breast cancer cell line MCF-7. There was a significant difference between 50μ mol/L group and control group on inhibition of MCF-7 migration （P 〈 0.05）. Moreover, it could cause the change of the expression of apoptosis related gene mRNA in ERK signaling pathway. Caspase-3 and caspase-9 were activated. There was a significant difference on Bcl-2 and Bax between the 50umol/L group and the control group （P 〈 0.05）. Conclusion Baicalin activates caspase-3 and caspase-9 through the ERK signaling pathway. Down regulation of Bel-2, up regulation of p-ERK, Bax, p38, it induces apoptosis of breast cancer MCF-7 cells. Baicalin can show greater economic benefits. What＇ s more, it can provide a new medicine and treatment for breast cancer patients.
出处
《中国医药科学》
2017年第5期14-18,共5页
China Medicine And Pharmacy
基金
广东省江门市科技计划项目
关键词
黄芩苷
信号通路
凋亡
Baicalin
Signaling pathway
Apoptosis
