摘要
由于链内碱基互补配对作用形成的"发夹"结构DNA分子被广泛用于生物分子传感分析。双链或多链"发夹"结构DNA分子参与的杂交链式反应信号记录方式多样,主要有荧光法、比色法、电化学方法等。基于杂交链式反应的检测方法具有快速、方便、成本低、准确度高、灵敏度高、特异性强的优点,在分析传感研究中的应用尤其受到人们的关注,近些年发展迅速。本文综述了"发夹"结构DNA与杂交链式反应应用于生物传感分析的原理、信号记录方式及其在蛋白质、重金属离子、小分子、疾病标志物、DNA等检测中的研究进展。
Owing to the complementary base pairing mechanism, the single strand DNA can form hairpin structure molecules, and is widely used in the biosensing and analysis. The signal recording modes of hybridization chain reaction （HCR） which involved two or more hairpin structure DNA include fluorescent analysis, colorimetric assay and electrochemical method, etc. Because of the advantages, such as fast, convenient, cost-efficient, accurate, sensitive and selective, the HCR based methods have attracted more and more attentions in biosensing and developed rapidly in recent years. Herein, we introduce the mechanisms of hairpin structure DNA and HCR used in the biosensing process, the signal recording methods and the applications in the detections of proteins, heavy metal ions, small molecules, disease biomarkers and DNA.
出处
《化学通报》
CSCD
北大核心
2017年第5期420-427,共8页
Chemistry
基金
国家自然科学基金项目(21305032)
中国博士后科研基金项目(2014M551522)
江苏省博士后科研基金项目(1402073B)
江苏大学高级人才启动基金项目(13JDG069)资助
